AllDocs -> cancers

Biochem. J. (1994) 302, 425-428 (Printed in Great Britain) Molecular cloning of a novel N-terminal variant of annexin 11 from rat basophilic leukaemia cells A. Louise UPTON and Stephen E. MOSS* *Department of Physiology, University College London, Gower Street, London WC1 E 6BT, U.K. Rat annexin II cDNA clones were isolated from a rat basophilic leukaemia cell plasmid library by cross-species hybridization with a mouse probe, and fully sequenced using the dideoxy- chain-termination method. Alignment ofthe derived amino-acid sequence with those of other mammalian annexin II species revealed a high level ofconservation, characteristic ofthe annexin family of proteins. One of the cDNAs isolated contained an additional six nucleotides close to the N-terminus, lying in-frame ...

Biochem. J. (1995) 305, 879-887 (Printed in Great Britain) Biphasic and differential modulation of Ca2+ entry by ATP and UTP in promyelocytic leukaemia HL60 cells Mayte MONTERO, Javier GARCIA-SANCHO* and Javier ALVAREZ Departamento de Bioquimica y Biologia Molecular y Fisiologfa, Facultad de Medicina, Universidad de Valladolid, 47005-Valladolid, Spain ATP and UTP cause mobilization of Ca2+ from the intracellular stores with similar potency in several cell types including both undifferentiated and differentiated HL60 cells. We show here that, in HL60 cells with Ca2+ stores that had been fully and irreversibly emptied using the endomembrane Ca2+-ATPase inhibitor thapsigargin, both nucleotides produced a biphasic effect ...

Biochem. J. (1990) 272, 259-262 (Printed in Great Britain) Induction of cyclo-oxygenase synthesis in human promyelocytic leukaemia (HL-60) cells during monocytic or granulocytic differentiation Atsushi HONDA,*T Amiram RAZt and Philip NEEDLEMANt *Department of Biochemistry, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, Japan, and tDepartment of Pharmacology, Washington University School of Medicine, St. Louis, MO 63110, U.S.A. Cyclo-oxygenase (COX) production in human promyelocytic leukaemia (HL-60) cells was studied during monocytic differentiation induced by la,25-dihydroxyvitamin D3 (24 nM; 3 days) or phorbol 12-myristate 13-acetate (100 nM; 1 day), or ...

Identification of two elements involved in regulating expression of the murine leukaemia inhibitory factor gene Li-Wei HSU* and John K. HEATHt CRC Growth Factor Group, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K. Mouse leukaemia inhibitory factor (LIF) is a polyfunctional has been shown to be hypomethylated in vitro [Kaspar, Dvorak cytokine which exhibits multiple functions in vitro and in vivo. and Bartunek (1993) FEBS Lett. 319, 159-162]. Analysis of the Two forms of LIF cDNA, differing at their 5' ends, have been LIF/neomycin-resistance transgene expression in these stable described encoding eitherdiffusible (D-LIF) ormatrix -associated cell clones demonstrated that transcripts containing the M-LIF (M-LIF) forms of the protein [Rathjen, ...

Biochem. J. (1993) 289, 761-766 (Printed in Great Britain) Control of Ca2+ entry into HL60 and U937 human leukaemia cells by the filling state of the intracellular Ca2+ stores Sara R. ALONSO-TORRE, Javier ALVAREZ, Mayte MONTERO, Ana SANCHEZ and Javier GARCiA-SANCHO* Departamento de Bioquimica y Biolog(a Molecular y Fisiolog(a, Facultad de Medicina, Universidad de Valladolid, 47005-Valladolid, Spain Differentiation of HL60 cells by treatment with dimethyl sulph- oxide induces the expression of membrane receptors for N- formylmethionyl-leucyl-phenylalanine (fMLP) and for platelet- activating factor (PAF). In these cells both agonists produced an increase in the cytosolic Ca2t concentration ([Ca2j]i) by re- lease of Ca2t from ...

Biochem. J. (1993) 296, 577-583 (Printed in Great Britain) Sulphydryl groups in the template-primer-binding domain of murine leukaemia virus reverse transcriptase IdentHication and functional analysis of cysteine-90 Subhalakshmi BASU, Amaresh BASU and Mukund J. MODAK* Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, U.S.A. Treatment of murine leukaemia virus reverse transcriptase with benzophenone 4-maleimide inactivates DNA polymerase ac- tivity, but has no effect on the RNAase H function. Kinetic measurements indicated that benzophenone 4-maleimide is a competitive inhibitor with respect ...

Biochem. J. (1993) 291, 303-307 (Printed in Great Britain) Phosphorylation of human topoisomerase I by protein kinase C in vitro and in phorbol 12-myristate 13-acetate-activated HL-60 promyelocytic leukaemia cells Elena CARDELLINI and Egon DURBAN* Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, U.S.A. Topoisomerase I was phosphorylated in vitro by protein kinase C (PKC) purified from rat brain with high affinity (Km about 0.1 ,M). Tryptic phosphopeptide mapping indicated that two major topoisomerase I peptides phosphorylated in vivo were co- migrating with minor peptides phosphorylated by PKC in vitro. INTRODUCTION Protein kinase ...

8 Report from the Acute Leukaemia Working Party Unrelated Cord Blood Transplants in Adults: A real alternative allogeneic hematopoietic stem cell source to treat patients with Acute Leukaemia To date more than 1000 unrelated cord blood transplants have been performed in adults with a unit coming from the Netcord organization. However the available information in this setting is still limited to small series of patients. In 1997, the Eurocord group published that the number of cord blood cells infused was the most important factor for engraftment and survival of cord blood transplant recipients in the New England Journal of Medicine (1). Since then, Netcord cord blood banks have been aware of the need to collect and freeze units large enough to accommodate adults and clinicians have also become aware of the importance of cell dose in cord blood transplants. Thanks to this ...

77 Imagens em Hematologia Rev. bras. hematol. hemoter. 2005; 27 (1):77-78 IMAGENS EM HEMATOLOGIA/IMAGES IN HEMATOLOGY Gustavo J. Lourenço 1 Lidiane C. Rueda 2 Manoela M. Ortega 3 Iramaia A. Néri 4 Rosemeire A.V. Bognone 5 Carmen S.P. Lima 6 Translocation t(1;16) in Chronic Eosinophilic Leukaemia Translocação t(1;16) em Leucemia Eosinofílica Crônica 1 Aluno de mestrado do Depto. de Clínica Médica da Faculdade de Ciências Médicas da Universidade Estadual de Campinas 2 Bióloga estagiária do Laboratório de Citogenética do Hemocentro da Universidade Estadual de Campinas 3 Aluna de doutoramento do Depto. de Clínica Médica da Faculdade de Ciências Médicas da Universidade Estadual de Campinas 4 ...

September, 2005 PRESS RELEASE TRUST RESEARCH COULD HELP LEUKAEMIA PATIENTS Researchers at Hull and East Yorkshire Hospitals NHS Trust are conducting a study into leukaemia , which may help to transform the lives of patients. A collaborative team, based in the Pathology Department at Hull Royal Infirmary and Hull University is hoping to shed some light on why some patients with the relatively common chronic lymphocytic leukaemia experience severe symptoms, while others experience virtually no symptoms at all. They hope that identifying differences between the two main types of leukaemia that this will pave the way for new treatments for this disease. The team is working in conjunction with the University of Hull to employ a state-of-the-art technique, mass spectrometry, which will allow them to look at individual molecules inside the leukaemia cells to establish their purpose. The study, ...

828 www.japi.org © JAPI • VOL. 53 • SEPTEMBER 2005 Correspondence Granulocytic Sarcoma as Initial Presentation of Acute Myeloid Leukaemia Sir, Acute myeloid leukaemia is a neoplastic proliferation of immature cells of haemopoietic system characterized by infiltration by these cells in blood, bone marrow and other tissues and is diagnosed by finding of blasts more than 30%, either in bone marrow or peripheral blood film (PBF). Myeloblastoma is a rare tumour of early myeloid precursor cells. Dock 1 was the first to note the association of it with acute leukaemia , and this tumour is regarded as an ominous harbinger of acute myelocytic leukaemia (AML), or the onset of blast crisis in chronic myelogenous leukaemia . Here, we are reporting a case who presented with subacute paraplegia due to a mass lesion (granulocytic sarcoma) without typical ...

MYELODYSPLASTIC SYNDROMES MEDICAL APPENDIX (PRELEUKAEMIA, SMOULDERING LEUKAEMIA ) DEFINITION 1. Myelodysplastic syndromes (MDS) are acquired, usually progressive, cytopenias associated with a hypercellular bone marrow and ineffective haemopoiesis. 2. Two major types are recognised: 2.1. Primary myelodysplastic syndromes , these being the more common. 2.2. Secondary myelodysplastic syndromes . CLINICAL MANIFESTATIONS 3. The most common presenting features are anaemia, fever or manifestations of bleeding. There are usually few, if any, significant physical signs. Splenomegaly is found in 20 per cent of cases. AETIOLOGY 4. Myelodysplastic syndromes almost always affect adults over the age of 50 years, the median age of patients with primary myelodysplastic syndromes being between 60 and 70 years. Secondary myelodysplastic syndromes as a rule affect younger ...

119 Chapter 11 Leukaemia Peter Adamson Summary • In the UK and Ireland, leukaemia accounted for about 1 in 40 diagnosed cases of cancer and 1 in 40 deaths from cancer. • Incidence was relatively high in the south of Scotland, Wales, parts of south east England, and parts of north east England, and relatively low in the north west and east of England. • The geographical pattern of mortality rates was broadly similar to that for incidence, although mortality was higher in Ireland and lower in Scotland than would have been expected from their incidence rates. • There is a suggestion of an inverse association between incidence and socio-economic deprivation, reflected in higher rates in some of the more affluent areas of the south of England and lower rates in the more deprived areas of the north. ...

leukaemia 1581 Dr Heather O Dickinson http://www.staff.ncl.ac.uk/heather.dickinson/ Department of Child Health University of Newcastle Cancer trends in England and Wales Deaths by cause (all ages), England & Wales, 1998 cancer 25% diseases of circulatory system 41% diseases of respiratory system 16% other 18% Age 1-4 yrs congenital anomalies Age 5-24 yrs accidents nervous system Age 25-74 yrs Over 75 yrs cancer respiratory system circulatory system other Deaths by cause and age group, 1998 5-year survival 0% 20% ...

Index acute myeloid leukaemia (AML) 396, 397–401 adult-onset Still’s disease (AOSD) 382 antigen presentation 44–5 apoptosis, in HLH 337–8 perforin role 340 benign cephalic histiocytosis (BCH) 141, 294–7, 304 bisphosphonate therapy 166 bone disease Erdheim–Chester disease (ECD) 19, 307–10 LCH 15–20, 95–103, 154–70 clinical aspects 154 clinical presentation 154–6 craniofacial bones 210–11, 215, 223, 277 late sequelae 274–5 orbits 116–17, 158–60, 169, 210–11 orthopaedic disabilities 274–5 reactivation of disease 169–70 skull base lesions 210–11 spine 156–7, 168 temporal bone 157–8, 168–9 radiologic investigations 160–5 bone scintigraphy 161–2 coincidence FDG PET scan 164–5 computerized tomography 162 magnetic resonance imaging 163 plain radiographs 160–1 therapy 165–7, 236–48 bisphosphonate therapy 166 chemotherapy ...

Abstract. Vincristine (VCR), a microtubule interfering anti- cancer agent, plays a key role in the treatment of childhood acute lymphoblastic leukaemia (ALL). The route of VCR induced apoptosis in ALL cells is not well defined. In this study we demonstrated caspase-9 and -3 activation in vivo in bone marrow leukaemic cells of a child with newly diagnosed ALL, after treatment with a single dose of VCR. We hypothe- sized that VCR induced apoptosis in ALL cells proceeds by a mitochondrial controlled pathway. We further studied the route of VCR induced apoptosis in Jurkat acute lymphoblastic leukaemia cells. First we showed that VCR induces activation of caspase-9 and -3 in Jurkat cells. With the caspase-9 inhibitor Z-LEHD-FMK we proved that caspase-9 was activated prior to caspase-3. Loss of mitochondrial transmembrane potential was independent of caspase-9 activation. To confirm the mitochondrial ...

a report by Professor Martin J S Dyer Professor of Haemato-Oncology and Honorary Consultant Physician, Medical Research Council (MRC) Toxicology Unit, University of Leicester Risk Stratification in the Treatment of Chronic Lymphocytic Leukaemia B U S I N E S S B R I E F I N G : E U R O P E A N P H A R M A C O T H E R A P Y 2 0 0 5 1 Reference Section Martin J S Dyer is Professor of Haemato-Oncology and Honorary Consultant Physician in the Medical Research Council (MRC) Toxicology Unit at the University of Leicester. His research interests are the antibody therapy and molecular genetics of B-cell malignancy. He worked for 11 years at the Royal Marsden Hospital with Professor Daniel Catovsky, following his training in medicine at Oxford and Cambridge Universities in the UK. Introduction One of the greatest medical ...

Understanding Caring for people with cancer acute myeloid leukaemia Page 2 The Irish Cancer Society would like to thank CancerBACUP–the British Association of Cancer United Patients – for their permission to adapt the text of their booklet entitled Understanding Acute Myeloid Leukaemia , published 2003. Page 3 This booklet has been written to help you understand acute myeloid leukaemia (AML). It has been prepared and checked by doctors, other relevant specialists, nurses and patients. It represents an agreed view on this leukaemia , its diagnosis and management, and the key aspects of living with it. Your hospital may have special guidelines and practices not mentioned in this booklet, but your doctor or nurse will explain these to you. If you are a patient, your doctor or nurse may go through the ...

Clinical and genetic studies of ETV6/ABL1-positive chronic myeloid leukaemia in blast crisis treated with imatinib mesylate Aikaterini Barbouti, 1 Tomas Ahlgren, 2 Bertil Johansson, 1 Mattias Höglund, 1 Carin Lassen, 1 Ingemar Turesson, 2 Felix Mitelman 1 and Thoas Fioretos 1 1 Department of Clinical Genetics, Lund University Hospital, and 2 Department of Medicine, Section of Haematology, Malmö University Hospital, Sweden Received 9 January 2003; accepted for publication 28 February 2003 Summary. Most chronic myeloid leukaemia (CML) patients are genetically characterized by the t(9;22)(q34;q11), gen- erating the BCR/ABL1 fusion gene. However, a few CML patients with rearrangements of 9q34 and 12p13, leading to ETV6/ABL1 chimaeras, have also been reported. ...

Chronic Myeloid Leukaemia 45 Chronic Myeloid Leukaemia : Molecular Abnormalities and Treatment Options Niamh Appleby, Eimear Burke, Terry-Ann Curran and Elaine Neary, 4 th Year Medicine INTRODUCTION Chronic myeloid leukaemia (CML) is a malignant myeloproliferative disorder of haemopoietic stem cells, giving rise to abnormally elevated numbers of myeloid cells in peripheral blood and myeloid hyperplasia in bone marrow. Uncontrolled production of myeloid precursors results in increased levels of maturing granulocytes, mainly neutrophils but also eosinophils and basophils. Elevated numbers of erythoid cells and platelets are also found. Clinical Features of CML Peripheral blood abnormalities account for many of the clinical features of CML (Table 1). Systemically the excess of neutrophils is accompanied by splenomegaly, and occasionally ...

23/05/05 Draft 7, Guidelines on the management of acute myeloid leukaemia in adults. British Committee for Standards in Haematology (BCSH) Milligan DW, Grimwade D, Cullis J O, Bond L, Swirsky D, Craddock C, Kell J, Homewood J, Campbell K, McGinley S, Wheatley K, G Jackson. Address for correspondence: BCSH Secretary British Society for Haematology 100 White Lion Street London N19PF e-mail jules@b-s-h.org.uk Review Date Jan 2008 Page 2 Master Draft 7 3 April 05 2 Page 3 Master Draft 7 3 April 05 3 Disclaimer. While the advice and information in these guidelines is believed to be true and accurate at the time of going to press, neither the authors, the British Society for Haematology nor the publishers accept any legal responsibility for the content of these guidelines. ...

Aetiology of Childhood Leukaemia Tracy Lightfoot Epidemiology & Genetics Unit Page 2 Childhood Cancer 0.0% 5.0% 10.0% 15.0% 20.0% 25.0% 30.0% 35.0% leukaemia brain lymphoma soft tissue sarcoma neuroblastoma kidney bone germ cell retinoblastoma epithelial liver other Page 3 What is Leukaemia ? • Leukaemia is a clonal disease originating in a single cell • It evolves by the accrual of mutations within a clone resulting in genetic diversification • Dominant mutant subclones are then naturally selected • The nature of the clone and how far it has evolved determines the clinical outcome • Delay in diagnosing increases the likelihood that the clone will have progressed ...

www.emergency.qld.gov.au EMERGENCYApril 2005 29 E mergency Services staff, volunteers, family and friends from all over Queensland raised thousands of dollars last month to support the Leukaemia Foundation’s annual World’s Greatest Shave For A Cure campaign. In Bundaberg, it was an emotional day for QAS Comms Officers following the recent passing of popular Communications Centre Supervisor, Andrew Penningh, who lost his battle with the debilitating disease in September 2004. Comms Officer Mel Bayntun said all Bundy staff felt a strong attachment to the cause and not many had escaped the barber’s clippers or spray can. Further north in Gladstone, paramedics Russell Thomas, Ben Ghee and Ian Brown courageously volunteered to walk 530km to Brisbane in just 14 days to show their support for the cause. The trio will ...

March 2005 CHRONIC MYELOGENOUS LEUKAEMIA CHRONIC MYELOGENOUS LEUKAEMIA 1 1 st st ALLOGENEIC TRANSPLANT (HLA ALLOGENEIC TRANSPLANT (HLA - - Identical sibling or unrelated donor) Identical sibling or unrelated donor) RELAPSE INCIDENCE ACCORDING TO THE PERIOD OF TRANSPLANT RELAPSE INCIDENCE ACCORDING TO THE PERIOD OF TRANSPLANT Relapse Incidence Years from transplant Years from transplant 14 14 12 12 10 10 8 8 6 6 4 4 2 2 0 0 .0 .0 .2 .2 .4 .4 .6 .6 .8 .8 1.0 1.0 Period of Period of No. No. No. No. Transplant Total Relapse HR (95%CI) Transplant Total Relapse HR (95%CI) 1992 1992 - - 96 96 3709 ...

REVIEW Nucleoside transporters in chronic lymphocytic leukaemia M Pastor-Anglada 1 , M Molina-Arcas 1 , FJ Casado 1 , B Bellosillo 2 , D Colomer 3 and J Gil 4 1 Departament de Bioquim i Biologia Molecular, Universitat de Barcelona, Barcelona, Spain; 2 Servei de Patologia, Hospital del Mar, Barcelona, Spain; 3 Unitat d’Hematopatologia, Hospital Clin IDIBAPS, Barcelona, Spain; and 4 Departament de Cie`ncies Fisiolo`giques II, Universitat de Barcelona, Hospitalet, Spain Nucleoside derivatives have important therapeutic activity in chronic lymphocytic leukaemia (CLL). Experimental evidence indicates that in CLL cells most of these drugs induce apoptosis ex vivo, suggesting that programmed cell death is the mechanism of their therapeutic ...

Keypoint blood. 20.12.01 -------------------------------------------------------------------------------------------------------------------------------------------------------------------------- DSMIG(UK) 2001. www.dsmig.org.uk 14.01.02 DOWN’S SYNDROME: BLOOD DISORDERS / LEUKAEMIA Key Points (based on conference paper by Professor Judith Chessells at RSM conference. April 2001) • Over 60% of neonates polycythaemic – unrelated to congenital heart disease • MCV increased at all ages • Almost unique risk of transient abnormal myelopoesis (TAM) in newborns (approx 10%) • Morphologically indistinguishable from megakaryocytic leukaemia • Usually asymptomatic • Usually resolves spontaneousely without treatment • Around 25% with TAM may develop acute myeloid leukaemia (AML) later in childhood ...

Childhood leukaemia , brain tumours and retinoblastoma near the Menai Straits, North Wales 2000-2003 A response to a recent Green Audit report: - “Nuclear pollution, childhood leukaemia , retinoblastoma and brain tumours in Gwynedd and Anglesey wards near the Menai Straits, North Wales 2000-2003” by C Busby PhD Ceri White MMath, John Steward PhD FFPHM, Rachel Wade BSc Welsh Cancer Intelligence and Surveillance Unit, Cardiff Background This internet report makes a number of claims concerning high risks of certain childhood cancers in North Wales. Part 1 makes reference to previous work carried out by Green Audit on downloads of tabulated data received from the Wales Cancer Registry (WCR) in 1996 covering the period 1982-1990. In part 2 of this report, data collected by HTV researchers concerning current cases are introduced and analysed in parts 3 to 5. Findings from the Green Audit ...

Leukaemia Introduction This has been prepared to help you understand more about leukaemia. There are several forms of leukaemia, so sometimes in this information we talk about ‘leukaemias’. Leukaemias are disorders of blood cell production which can occur in people of any age. Many people feel understandably shocked and upset when they are told that they have leukaemia. This information is intended to help you understand the diagnosis and treatment of the disease. We also include information about available support services. We cannot advise you about the best treatment for you. You need to discuss this with your own doctors. However, we hope this information will answer some of your questions and help you in thinking about the questions you want to ask your doctors. What is Cancer? Cancer is a disease of the body’s cells. Our bodies are constantly making new cells: to enable us to grow, ...

Is indoor radon linked to leukaemia in children and adults? – A review of the evidence D. L Henshaw and J. E Allen H H Wills Physics Laboratory, University of Bristol, Tyndall Avenue, Bristol, BS8 1TL, UK, E-mail: d.l.henshaw@bris.ac.uk Abstract The evidence linking indoor radon exposure to childhood and adult leukaemia is re- viewed. At the UK average indoor exposure of 20 Bq m -3 , it is estimated that the radon de- rived equivalent dose accrued to the fetus is 106 µSv. The equivalent dose rate to adult bone marrow is 130 µSv y -1 . Standard radiation risk factors suggest that 5% of childhood and 4% of adult leukaemia is linked to radon at 20 Bq m -3 exposure. Geographical studies generally support such a link at about this magnitude. A number of case-control studies have been carried out but these in general have not had enough ...