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Scrapie prion protein accumulation by scrapie- infected neuroblastoma cells abrogated by exposure to a prion protein antibody Masato Enari, Eckhard Flechsig, and Charles Weissmann* Medical Research Council Prion Unit, Neurogenetics, Imperial College School of Medicine at St. Mary’s, London W2 1PG, United Kingdom Contributed by Charles Weissmann, May 15, 2001 Exposure of susceptible neuroblastoma N2a cells to mouse scrapie prions leads to infection, as evidenced by the continued presence of the scrapie form of the prion protein (PrP Sc ) and infectivity after 300 or more cell doublings. We find that exposure to phosphati- dylinositol-specific phospholipase C (PIPLC) or to the monoclonal anti-prion protein (PrP) antibody 6H4 not only prevents infection of susceptible N2a cells but also cures chronically scrapie-infected cultures, as judged by the long-term ...

Mini-review High-resolution cDNA microarray-based comparative genomic hybridization analysis in neuroblastoma Qing-Rong Chen 1 , Sven Bilke 1 , Javed Khan* Oncogenomics Section, Pediatric Oncology Branch, Advanced Technology Center, National Cancer Institute, 8717 Government Circle, Gaithersburg, MD 20877, USA Received 24 November 2004; accepted 14 December 2004 Abstract Neuroblastoma (NB) is one of the most common pediatric solid tumors and displays a broad variety of genomic alterations. Array-based comparative genomic hybridization (A-CGH) is a novel technology enabling the high-resolution detection of DNA copy number aberrations. In this article, we outline features of this new technology and approaches of data analysis. We focus on stage specific DNA copy number variations in neuroblastoma detected by cDNA array-based comparative genomic hybridization ...

Inferring a Tumor Progression Model for Neuroblastoma From Genomic Data Sven Bilke, Qing-Rong Chen, Frank Westerman, Manfred Schwab, Daniel Catchpoole, and Javed Khan From the Oncogenomics Section, Pediatric Oncology Branch, Advanced Technology Center, National Cancer Institute, Gaithersburg, MD; Department of Tumor Genetics, German Cancer Research Center, Heidelberg, Germany; and Tumour Bank, The Children’s Hospital at Westmead, Westmead, Australia. Submitted June 27, 2005; accepted July 18, 2005. Supported in part by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research. S.B. and Q.-R.C. contributed equally to this work. Authors’ disclosures of potential conflicts of interest are found at the end of this article. Address reprint requests to Javed Khan, ...

Original article Meta -[ 123 I]iodobenzylguanidine is selectively radiotoxic to neuroblastoma cells at concentrations that spare cells of haematopoietic lineage Ying He a , Bikul Das b , Sylvain Baruchel b , Piyush Kumar c , Leonard Wiebe c and Raymond M. Reilly a,d,e Background The Auger electron-emitting agents meta- [ 125 I]iodobenzylguanidine ( 125 I-MIBG) and 123 I-MIBG have been proposed as alternatives to 131 I-MIBG for the treatment of neuroblastoma , due to the absence of a cross- fire effect which may minimize bone marrow toxicity. However, the differential toxicity of 123 I-MIBG towards neuroblastoma cells and cells of haematopoietic lineage has not been studied. ...

Enhanced Activity of Hu14.18-IL2 Immunocytokine against Murine NXS2 Neuroblastoma when Combined with Interleukin 2 Therapy Zane C. Neal, 1 Jeannie C. Yang, 1 Alexander L. Rakhmilevich, 1 Ilia N. Buhtoiarov, 1 Hillary E. Lum, 1 Michael Imboden, 2 Jacquelyn A. Hank, 1 Holger N. Lode, 3 Ralph A. Reisfeld, 4 Stephen D. Gillies, 5 and Paul M. Sondel 1 1 Department of Human Oncology, University of Wisconsin–Madison, Madison, Wisconsin; 2 IoGenetics, Middleton, Wisconsin; 3 Charite Children’s Hospital, Humboldt University, Berlin, Germany; 4 Department of Immunology, The Scripps Research Institute, La Jolla, California; and 5 ...

THE NEUROBLASTOMA SOCIETY 1 GRANT APPLICATION FORM 1. Applicant(s) Applicant 1 Applicant 2 Applicant 3 Forename(s) Title Post held Department/Institution No of hours p/w on project 2. Address of Institution/Authority 3. Address where the work will be done: (Administering grant if approved): __ 4. Title of Research (do not exceed 120 characters including spaces): 5. Type of grant sought: __ 6. Scientific abstract of research (in no more than 250 words): 7. SUMMARY OF SUPPORT REQUESTED (see Section 16) YEAR 1 £ YEAR 2 £ YEAR 3 £ TOTALS £ Salaries Consumables Travel & Subsistence Exceptional Items Equipment TOTAL COST - - - 8. Proposed starting date: __ 9. Proposed ...

Translational Genomics in Neuroblastoma (TGIN) Meeting Proposed Agenda DAY DATE BEGIN END EVENT Sunday 10/16/2005 3 PM 5 PM Registration 5 PM 6 PM Posters 6 PM Begin Dinner 7:00 PM 7:15 PM Welcome 7:15 PM 7:45 PM Plenary Session 8:00 PM 8:30 PM Plenary Session 8:45 PM 9:15 PM Plenary Session Monday 10/17/2005 6:30 AM Breakfast begins 7:00 AM 12:00 PM Session 1 1:00 PM 5:00 PM Session 2 5:00 PM 6:00 PM Poster/Dinner 7:30 PM 9:30 PM Session 3 Tuesday 10/18/2005 6:30 AM Breakfast begins 7:00 AM 12:00 PM Session 3 1:00 PM 3:00 PM Round Table Discussion 3:00 PM END

Tribromophenol induces the differentiation of SH-SY5Y human neuroblastoma cells in vitro J.C. Rió a, b ,G. Repetto a, c, * ,A. Jos a ,A. del Peso a ,M. Salguero a ,A. Cameán c , M. Repetto a, c a National Institute of Toxicology, PO Box 863, 41080, Sevilla, Spain b CITUC, School of Medicine, Pontificia Universidad Católica de Chile, Chile c Area of Toxicology, Universidad de Sevilla, Sevilla, Spain Accepted 31 May 2003 Abstract Tribromophenol is a pesticide with fungicide activity,presently used as a replacement of pentachlorophenol as a wood pre- servative,and as a flame retardant in electronic and electrotechnical devices. Retinoic acid differentiated and non-differentiated SH- SY5Y human neuroblastoma cell cultures were ...

N Engl J Med, Vol. 346, No. 14 · April 4, 2002 · www.nejm.org · 1047 NEUROBLASTOMA SCREENING NEUROBLASTOMA SCREENING AT ONE YEAR OF AGE F REIMUT H. S CHILLING , M.D., C LAUDIA S PIX , P H .D., F RANK B ERTHOLD , M.D., R UDOLF E RTTMANN , M.D., N ATALJA F EHSE , M.D., B ARBARA H ERO , M.D., G ISELA K LEIN , P H .D., J OHANNES S ANDER , M.D., K ERSTIN S CHWARZ , M.D., J OERN T REUNER , M.D., U LRICH Z ORN , P H .D., AND J OERG M ...

Biochem. J. (2005) 386 , 291–296 (Printed in Great Britain) 291 Activation of ryanodine receptors induces calcium influx in a neuroblastoma cell line lacking calcium influx factor activity Diptiman D. BOSE, Roshanak RAHIMIAN and David W. THOMAS 1 Department of Physiology and Pharmacology, Thomas J. Long School of Pharmacy and Health Sciences, University of the Pacific, Stockton, CA 95211, U.S.A. We have further characterized the Ca 2+ signalling properties of the NG115-401L (or 401L) neuroblastoma cell line, which has served as an important cell line for investigating SOC (store- operated channel) influx pathways. These cells possess an unusual Ca 2+ signalling phenotype characterized by the absence of Ca 2+ influx when Ca 2+ stores are depleted by inhibitors of SERCA (sarcoplasmic/endoplasmic reticulum Ca ...

Rev. 0412.1 P RESENTATION Anti- Neuroblastoma (NB84a) is a mouse monoclonal antibody, diluted in phosphate buffered saline, pH 7.6, with 1% bovine serum albumin, and preserved with sodium azide. A PPLICATIONS Anti-NB84a is a monoclonal antibody produced using human neuroblastoma tissue as a source of antigen. It recognizes an uncharacterized 57 kD molecule. It is useful in identifying neuroblastoma (99%(+)), and desmoplastic small round cell tumors (50%(+)). A small percentage of Ewing’s sarcoma (20%) and rhabdomyosarcomas (20%) stain positive with this antibody. CE Partners 4U 395 1 DB, 13 NL +31 (0) 6-515.536.26 Paraffin, Frozen Neuroblastoma Cytoplasmic Up to 36 months; store at 2-8°C IgG 1 A VAILABILITY D ESCRIPTION C ATALOG N O . D ...

NBL 07/09/01 Page 1 of 2 Study Summary ANBL00P2 Perinatal Neuroblastoma : Expectant Observation: A COG Pilot Study This study evaluates treatment for infants with a cyst or small mass in the adrenal gland (a gland found over each kidney that secretes hormones). In many cases such a mass is a tumor called neuroblastoma , although it could also represent a lung malformation called pulmonary sequestration, or a blood clot in the adrenal gland, referred to as adrenal hemorrhage. The current standard treatment for neuroblastoma is surgical removal of the tumor. We have learned a great deal about this tumor recently. One of the key findings is that it behaves quite differently in infants compared to older children. Several recent studies have suggested that anywhere between 33 and 100% of these small adrenal tumors in young infants spontaneously resolve (disappear on their own, ...

46.00 CASE REPORT - NEUROBLASTOMA OF FRONTAL CEREBRAL LOBE - 8 YEARS AFTER INTENSIVE THERAPY K. Bajerova , M. Bajer, H. Hrstkova 1 st Pediatric Clinic, Brno, Czech Republic mibaja@tiscali.cz History: The baby was born after a physiological pregnancy. Short after birth the baby has presented general convulsions. The CT scan of the CNS has discovered a neuroblastoma located in the left frontal lobe. An operation, complete removal of the tumour, was followed by a chemotherapy consisting of NB-A-89 protocol. No severe complications have occurred during the therapy, except anaemia and few respiratory infections. The clinical follow up went on after finishing therapy. Blood tests – no evidence of abnormities CT scan of CNS – cavity in position of left frontal lobe communicating with first chamber. No presence of tumour. Psychomotor development – psychomotorial ...

D ATA S HEET Rev 030502D Neuroblastoma Marker Ab-1 (Clone NB84a) Mouse Monoclonal Antibody Cat. #MS-492-S0, -S1, or -S (0.1ml, 0.5ml, or 1.0ml Supernatant) Cat. #MS-492-R7 (7.0ml) (Ready-to-Use for Immunohistochemical Staining) Cat. #MS-492-PCS (5 Slides) (Positive Control for Histology) #&&( ÃXrvtu?rÃ9? ÃA?r?Ã86ÃÃ(#$"(ÃVT6Ã Ury)Ã ' Ã'!' %!'ÃÃAh?)Ã $ Ã(( !'!%Ã @?hvy)Ãyhi?v?v?5yhi?v?v? pÃÃXri?v?r)Ã yhi?v?v? p Comments: Ab-1 recongizes a protein of 57kDa which is found in a wide range of normal tissues, including most epithelia and endothelial cells. Ab-1 is useful in identification of pediatric, small round blue cell tumors. Approximately 90% of neuroblastomas are stained by this antibody whereas all other tumor types such as leukemia, rhabdomyosarcomas, and Wilm’s ...

1 DIAGNOSTIC IMAGING IN NEUROBLASTOMA Hans Ringertz, M.D., Ph.D. INTRODUCTION Neuroblastoma is a biologically very special neoplasm that is unique to child- hood. As can be seen from Table 1 it is the fourth most common paediatric malignancy in the Nordic countries and constitutes around 6% of all malignancies in children. Neoplasm in patients below 16 years of age in the Nordic countries during the years 1985 and 2001 in order of frequency No. of new cases % 1 Leukaemia 3249 34 2 Central nervous system and intracranial and intraspinal neoplasm 2596 27 3 Lymphomas and other reticuloendothelial neoplasm 1116 11 4 Sympathetic nervous system tumours 595 6 5 Renal tumours 556 6 6 Soft tissue sarcomas 540 5 7 Malignant bone tumours 297 3 8 Germ-cell, trophoblastic ...

Introduction Cyclopentenyl cytosine (CPEC) is an inhibitor of CTP synthetase and possesses anti-tumour activity against neuroblastoma in vitro (1, 2) . Incubation with CPEC depletes the (deoxy)cytidine nucleotide pools, and causes S-phase accumulation (1, 2). This makes the combination of CPEC with deoxynucleoside analogues attractive for chemotherapy. CPEC proved to be an excellent modifier of the deoxycytidine analogue cytarabine in both neuroblastoma and leukaemic cells (3, 4). 2-Chloro-2’deoxyadenosine or Cladribine (CdA) is an analogue of deoxyadenosine and is used in the treatment of haematological malignancies. However, no anti-tumour activity of CdA against solid tumours has been observed in clinical trials. CdA is an in- hibitor of DNA synthesis as well as DNA repair. It is also a potent inhibitor of ribonucleotide reductase, which is the primary source of deoxynucleotides ...

Biochem. J. (1996) 318 , 1033–1039 (Printed in Great Britain) 1033 Agonist regulation of adenylate cyclase activity in neuroblastoma i glioma hybrid NG108-15 cells transfected to co-express adenylate cyclase type II and the ß 2 -adrenoceptor Evidence that adenylate cyclase is the limiting component for receptor-mediated stimulation of adenylate cyclase activity David J. M AC EWAN, Gun-Do KIM and Graeme MILLIGAN* Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K. Stable cell lines, derived from NG108-15 cells and transfected to express both the ß # -adrenoceptor and adenylate cyclase type II, were produced and examined. The absence of adenylate cyclase type II in the parental cells and its presence in these ...

Section 4 – Your Child’s Diagnosis – Neuroblastoma Page 1 of 3 Neuroblastoma What is a Neuroblastoma ? Neuroblastoma is a tumour that begins in nerve tissues in the neck, chest, abdomen, or pelvis. About 50% of neuroblastomas start in the tissues of the adrenal glands located just above the kidneys. Often this tumour has spread before it is diagnosed. The common sites are the lymph nodes, liver, bones, and bone marrow. Neuroblastoma occurs in early childhood with 2/3 of the children younger than 5 years of age when they are diagnosed. Some neuroblastomas in infants can spontaneously regress and your doctor will discuss this with you. What causes a neuroblastoma ? As with most cancers, the cause of neuroblastoma is unknown. What are the signs and symptoms? The symptoms will vary depending on where the tumour is located. The most common ...

Monoclonal Antibody-based Therapy for Neuroblastoma Nai-Kong V. Cheung, MD, PhD Address Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA. E-mail: cheungn@mskcc.org Current Oncology Reports 2000, 2 :547 –553 Current Science Inc. ISSN 1523–3790 Copyright © 2000 by Current Science Inc. Introduction Two decades ago, patients with stage 4 neuroblastoma (NB) diagnosed at 2 years old or later had a dismal prognosis [1]. Faced with wide metastases to bone and marrow, treatment strategies were often of palliative intent. The sophisticated use of chemotherapy can now reduce bulky tumors to the minimal residual disease state (MRD) in most patients. Unfortunately, the transition from MRD to cure remained a formidable hurdle. Myeloablative therapy (ABMT) takes advantage of the steep dose- response ...

LABORATORY OF BIOCHEMICAL GENETICS Neuroblastoma and related lines of cultured cells were used as model systems for studies on synapse formation and other aspects of neuronal development. Prolonged elevation of cyclic AMP levels of neuroblastoma cells has profound, long-lived, stimulatory effects on the ability of the cells to form synapses and transmit information to other cells, which is due, in part, to the appearance of functional voltage-sensitive calcium channels. cDNA and genomic DNA clones were obtained that correspond to the a-subunit of L-type voltage-sensitive calcium channels of rat brain. In addition, seventeen cDNA clones were obtained ...

Toxicoiogy in Vitro 1 l (1997) 5 19-525 Morphological, Biochemical and Molecular Effects of Cocaine on Mouse Neuroblastoma Cells Culture In Vitro G. REPETTO*t, A. del PESO?, A. GARFIAt, M. J. GONZALEZ-MUfiOZS, M. SALGUERO& P. SANZt and M. REPETTOt tNational Institute of Toxicology, PO Box 863, 41080, Seville SDepartamento de Nutrition y Bromatologia, Facultad de Farmacia, Universidad de Alcala de Henares, Madrid and @Departamento de Citologia e Histologia Normal y Patolbgica, Facultad de Medicina, Universidad de Sevilla, Seville, Spain Abstract-In order to compare the effects of cocaine at morphological, basal cytotoxicity, biochemical and molecular levels, cultured mouse neuroblastoma cells (Neuro-2a) were exposed ...

MEDIA RELEASE April, 2005 New development in fight against childhood cancer Researchers at the Flinders Medical Centre have discovered a way to ‘switch off’ drug resistant genes to increase the chances of survival among children diagnosed with blood- related cancers. The research team, lead by Dr Bryone Kuss from the Department of Haematology and Genetic Pathology, has explored the role of certain drug resistant genes in neuroblastoma tumour cells and is investigating new approaches for the treatment of both neuroblastoma and acute leukemia. “It is not uncommon for cancers to become resistant to chemotherapy or radiotherapy and ultimately lead to cancer relapse,” Dr Kuss said. The team’s aim is to understand the mechanism behind the resistance in the cancers, which predominately affect children from birth. Although cure of acute leukemia is achievable in approximately ...

Negative Regulation of Neuroblastoma Cell Growth by Carbohydrate-dependent Surface Binding of Galectin-1 and Functional Divergence from Galectin-3* Received for publication, June 5, 2001, and in revised form, July 12, 2001 Published, JBC Papers in Press, July 12, 2001, DOI 10.1074/jbc.M105135200 Jürgen Kopitz‡, Carolina von Reitzenstein‡, Sabine André§, Herbert Kaltner§, Johannes Uhl‡, Volker Ehemann ¶ , Michael Cantz‡ , and Hans-Joachim Gabius§ From the ‡ Institut für Pathochemie und Neurochemie and the ¶ Pathologisches Institut, Klinikum der Ruprecht-Karls- Universität, Im Neuenheimer Feld 220, D-69120 Heidelberg, Germany and the § Institut für Physiologische Chemie, Tierärztliche Fakultät, Ludwig-Maximilians-Universität, Veterinärstr. 13, D-80539 München, Germany The cell density-dependent growth inhibition of hu- man SK-N-MC neuroblastoma ...

I81 " NEUROBLASTOMA -SCREENING IN GERMANY – A UNIQUE TRIAL F. Berthold, B. Hero, F.H. Schilling, R. Erttmann, C. Spix, R. Spitz, Universitätskinderklinik Köln (Köln) Prerequisites for the German Neuroblastoma Screening Project, which started in 1995, seemed favorable: 1) Great differences were seen between the prognosis of localized disease (10-year-survival about 80%) and metastatic disease (10-year-survival about 20%). 2) Detection of catecholamine metabolites (VMA and HVA) in urine is easy to carry out, is not very stressful for the children and has been proved useful in pilot studies (1991 – 1995). 3) Correlation of age and stage (stage 1: median age 13 months, stage 2: 15 months, stage 3: 17 months, stage 4: 33 months), the absence of stage 1 and 2 disease in developing countries and the long latency from localized disease to metastatic relapses (median 13 months) were considered strong, though only indirect ...

!"#$ P O ! VP S NMU ABSTRACT A child with neuroblastoma was hospitalized due to fever on November 6, 2003. During the hospitalization (11/06/ 2003-12/06/2003), the peripheral stem cells were collected from this child. The caring duration of the author was from November 20, 2003 through November 26, 2003. This article describes the medical and nursing interventions imple- mented for solving this child's health problems. As Gordon's 11 Functional Health Assessment served as an assessment guide, four health problems, such as risk for infection, altered oral mucous membrane, hyperthermia, and pain, were identified. In order to solve four health problems mentioned above, nursing goals were first identified and nursing inter- ventions were then implemented. After evaluating the nursing care planed for this child, four health problems were either improved or solved. This care experience may provide health ...

Research Reagents Mouse Neuroblastoma Cell Line N2a Overview Clone N2a was established by R.J Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse (ref. 1). This tumor line, designated C1300, was obtained from Jackson Laboratories, Bar Harbor, Maine. This neuroblastoma cell line is susceptible to prion infection and we offer this cell line as a control for the scrapie infected ScN2a cells (Product No. ICL-N2AI). Product Description Product No. ICL-N2AC Organism Mus musculus (mouse) Biosafety Level 1 Tissue Nerve Karotype 2n = 40 Genotype albino Morphology and Growth Neuronal and amoeboid stem cells. A cloned continuous culture, grown as a monolayer. Culture Conditions Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum (FBS), 2 mM L-glutamine, penicillin and ...