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neuroblastoma THE NEUROBLASTOMA SOCIETY The Society prepares the following formal report and financial statements in accordance with its duties under the Charities Act, and also publishes an Annual Report for the general reader. Both are produced here. REPORTS AND FINANCIAL STATEMENTS For the year ended 31 December 2003 CONTENTS Page Trustees’ annual report 2 - 3 Auditor’s report 4 Income and expenditure account 5 Statement of financial activities 6 Balance sheet 7 Notes to the financial statements 8 – 10 Annual Report 11 - 17 THE NEUROBLASTOMA SOCIETY - TRUSTEES’ REPORT TRUST DEED AND OBJECTIVES The Neuroblastoma Society was established ...

Proteome analysis of neuroblastoma cell line SH-SY5Y by GeLC-MS/MS and 2-DE. Gilany K, Van Elzen R, Van Dongen W, Dewilde S. , Moens L. Department of Biomedical Sciences, University of Antwerp, Antwerp Belgium. Each eukaryotic cell in a given organism contains the same genome but the protein content of cells varies widely, depending on cell-type, developmental stage, environmental conditions etc. Proteome analysis is usually performed by the separation of a protein mixture by two dimensional (2DE) gel electrophoresis and the identification of the separated proteins by mass spectrometry. Next to the fact that 2DE suffers from being labor intensive and that only high abundant proteins can be visualized, the detection of basic, hydrophobic and low molecular weight proteins is inefficient and the complex protein mixture exceeds the resolving power of 2DE. A simpler method for the proteome analysis is separation of the protein mixture ...

Caspase 2 3 and survivin expression in pediatric neuroblastoma and their roles in apoptosis WAN GJia2xiang ? and ZHEN G Shu ? ? Keywords : caspase 2 3 · survivin · neuroblastoma · TUNEL · ap optosis · in situ hybridization Cancer Institute , Second Affiliated Hospital , Zhejiang University , Hangzhou 310009 , China (Wang J X and Zheng S) Correspondence to : Dr 1 Wang Jia 2 xiang , Department of Pediatric Surgery , First Affiliated Hospital , Zhengzhou University , Zhengzhou 450052 , China ( Tel : 86 2 371 2 6862132 1 Fax : 86 2 371 2 6862133) Background Neuroblastoma , one of the common tumors in children , possesses ...

IDENTIFICATION OF A SPECIFIC INHIBITOR TO HERPES VIRUS TYPE 2 IN NEUROBLASTOMA CELLS Binie V. Lipps, Ophidia Products Inc. 11320 South Post Oak Suite 203 Houston, Texas 77035, USA. SUMMARY The results of this investigation showed a retardation of HSV-2 replication in neuroblastoma (NB) cells without affecting HSV-1 replication. HSV-1 and HSV-2 were grown in Chang's liver (CH), human embryonic lung (HEL), Vero and NB cells with same virus stocks. The harvests of the infected cells were assayed for infectivity titer in Vero cells. It was observed that the infectivity titer of HSV-2 exclusively in NB cells was three to four logs lower in comparison to CH, HEL and Vero cells. The clinical isolates of HSV-2 showed lower infectivity in NB cells in comparison to CH and HEL cells. The kinetics of replication of HSV-2 in NB cells showed retardation in comparison to HSV-1. This may ...

I78 NEUROBLASTOMA SCREENING STUDY ENABLES ADEQUATE DECISION ON FURTHER DEVELOPMENT OF LEGISLATIVE EARLY DETECTION PROGRAMME B. Metzinger, IKK-Bundesverband (Bergisch Gladbach) Studies about the effectiveness of screening are rare and far between in Germany. Realization of such studies usually is declined because of alleged ethical reasons. Consequently screening examinations are executed without previous effectiveness checks and quality management. Especially examinations in healthy individuals require evidence for their benefit. Necessarily the risk (including the risk of further examination) vs. benefit (better prognosis for early detected diseases) have to be weighed. The pilot project " Neuroblastoma screening" is a positive example for the intention of all persons concerned realizing a study to proove the benefit of a screening test. The result of the project answers the question whether screening ...

C y t o t o x i c i t y S c r e e n i n g N E U R A L N O T E S I S S U E 2 1 2 0 0 2 I N VITRO C YTOTOXICITY S CREENING OF H UMAN N EUROBLASTOMA C ELLS by Randy Hoffman, B.S., and Megan Buros, Promega Corporation Introduction Robotic integration of cell-based assays for in vitro toxicity screening of chemical compounds is becoming an increasingly important aspect of the drug discovery process. Development of assays that simplify the automation process is crucial for the success of this high- throughput screening. Promega has recently introduced the CytoTox-ONE™ Homogeneous Membrane Integrity Assay (a) , a fluorometric method for estimating the number of non-viable cells present in multiwell plates. Combining ...

N Engl J Med, Vol. 346, No. 14 · April 4, 2002 · www.nejm.org · 1047 N E U R O B L ASTO M A S C R E E N I N G NEUROBLASTOMA SCREENING AT ONE YEAR OF AGE F REIMUT H. S CHILLING , M.D., C LAUDIA S PIX , P H .D., F RANK B ERTHOLD , M.D., R UDOLF E RTTMANN , M.D., N ATALJA F EHSE , M.D., B ARBARA H ERO , M.D., G ISELA K LEIN , P H .D., J OHANNES S ANDER , M.D., K ERSTIN S CHWARZ , M.D., J OERN T REUNER , M.D., U LRICH Z ORN , P H .D., AND ...

PEDIATRIC GRAND ROUNDS THURSDAY, OCTOBER 14, 2004 8:00 TO 9:00 A.M. SPARROW HOSPITAL AUDITORIUM TARGETED RADIOTHERAPY FOR NEUROBLASTOMA USING 131I-MIBG GREGORY A. YANIK, M.D. Associate Professor Pediatric Hematology/Oncology Blood and Marrow Transplant Program University of Michigan

1 DIAGNOSTIC IMAGING IN NEUROBLASTOMA Hans Ringertz, M.D., Ph.D. INTRODUCTION Neuroblastoma is a biologically very special neoplasm that is unique to child- hood. As can be seen from Table 1 it is the fourth most common paediatric malignancy in the Nordic countries and constitutes around 6% of all malignancies in children. Neoplasm in patients below 16 years of age in the Nordic countries during the years 1985 and 2001 in order of frequency No. of new cases % 1 Leukaemia 3249 34 2 Central nervous system and intracranial and intraspinal neoplasm 2596 27 3 Lymphomas and other reticuloendothelial neoplasm 1116 11 4 Sympathetic nervous system tumours 595 6 5 Renal tumours 556 6 6 Soft tissue sarcomas 540 5 7 Malignant bone tumours 297 3 8 Germ-cell, trophoblastic ...

Changes in G1-phase populations in human glioblastoma and neuroblastoma cell lines influence p(66)/Be neutron-induced micronucleus yield John M. Akudugu a, * , Anke Binder a , Antonio Serafin a , Jacobus Slabbert b , Alf Giese c , Lothar Böhm a a Department of Radiation Oncology, Faculty of Health Sciences and Tygerberg Hospital, University of Stellenbosch, Tygerberg, South Africa b Radiation Physics Division, iThemba LABS, Faure, South Africa c Laboratory for Brain Tumour Biology, Department of Neurosurgery, University Hospital Eppendorf, Hamburg, Germany Received 16 September 2003; accepted 13 January 2004 Abstract Some photon resistant tumours are sensitive to neutrons but no predictive methods exist which could identify such tumours. In a recent study ...

Stimulation of using a Human Neuroblastoma Cells Planar Microelectrode Array Page 2 A thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the University of Glasgow. October 2000 Carolyn Audrey McEwan Centre for Cell Engineering University of Glasgow Stimulation of using a Human Neuroblastoma Cells Planar Microelectrode Array

Abstract. Valproic acid (VPA) as a differentiation inducing anti-neoplastic substance is currently tested in solid tumour and leukaemia patients. Previously, we were able to show that the anti-cancer activity of VPA was synergistically increased by interferon- · (IFN- · ) in Be(2)-C neuroblastoma (NB) cells. Now, we studied the effects of VPA in combination with IFN- · on two other NB cell lines. UKF-NB-2 and UKF-NB-3 cell growth was synergistically inhibited by VPA and IFN- · . Cell cycle investigations revealed massive accumulation of cells in G 0 /G 1 -phase after a combined treatment with VPA and IFN- · . The VPA-induced accumulation of acetylated histones in NB cell nuclei that indicates inhibition of histone deacetylases was not further enhanced by the combination treatment ...

Abstract. The majority of high-risk neuroblastomas lack the expression of caspase-8 due to gene silencing which suggest a mechanism for the selection of tumour cells that are refractory to multiple cytotoxic drugs including tumour necrosis factor- related apoptosis-inducing ligand (TRAIL). Inhibitors of DNA methyltransferases and IFN- Á induce expression of caspase-8, and sensitise some neuroblastoma cells to TRAIL-mediated apoptosis. Here we demonstrate that a combination of cyto- static drugs with IFN- Á and TRAIL synergistically induces neuroblastoma cell death, which may have implications for future therapy of children with neuroblastoma . Treatment of neuroblastoma cells with IFN- Á induced caspase-8 expression in all cell lines investigated. In five of the neuroblastoma cell lines (SHEP-1, SK-N-AS, SK-N-FI, SH-SY-5Y and Kelly), ...

Abstract. Neuroblastoma , a form of neuroblastic tumor, is one of the most common neoplasms seen in early childhood. The diverse clinical behavior of this tumor is most probably explainable by the heterogeneity in the associated genetic changes. We investigated 12 neuroblastoma patients for MYCN amplification and chromosome 2 aneusomy by fluorescence in situ hybridization (FISH) and results were correlated with conventional cytogenetics. Samples from both primary tumor tissue and bone marrow metastasis were available in two cases. The copy number of MYCN oncogene paralleled that of chromosome 2 in 10 cases, whereas two cases (16.7%) showed numerous distinct signals within the nuclei of the tumor cells, consistent with MYCN amplification as double minute (dmin). The morphology of dmin in one case was of an extremely small type and might potentially be missed by conventional chromosome analysis. ...

Review A Systematic Review of Molecular and Biological Tumor Markers in Neuroblastoma Richard D. Riley, 1 David Heney, 2 David R. Jones, 1 Alex J. Sutton, 1 Paul C. Lambert, 1 Keith R. Abrams, 1 Bridget Young, 3 Alan J. Wailoo, 4 and Susan A. Burchill 5 Departments of 1 Health Sciences, 2 Medical Education, University of Leicester, Leicester; 3 Department of Psychology, University of Hull, Hull; 4 School of Health and Related Research, University of Sheffield, Sheffield; and 5 Cancer Research United Kingdom Clinical Centre, St. James’s University Hospital, Leeds, United Kingdom Abstract Purpose: The aim of this ...

15 Cadmium-Induced Apoptosis in MES 23.5 Mouse Neuroblastoma Cells Presenter Wei-Wei Cheng Collaborators Li-xin Liu, Angela Gentles, Jean Strhlendrof, Louise Chiodo and Ernest Smith, Texas Tech University Department Cell and Molecular Biology Abstract Exposure to the heavy metal, cadmium (Cd2+), has been linked to adverse environmental and human health effects. The present studies addressed the mechanisms of Cd2+-induced cell death in the mouse neuroblastoma X rat mesencephalon (MES-23.5) wild type cell line. Toxicity initially was indexed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide (MTT) assay following exposure to Cd2+ (1-10_M) for 7 to 72 h. Cell death was observed to be both concentration- and time-dependent. The highest concentration resulted in 100% cell death following 72 h exposure via the culture medium. We directly evaluated ...

Dear Lunch for Life Sponsor, The Children’s Neuroblastoma Cancer Foundation would like to give you the opportunity to be one of our sponsors for the 2004 Lunch for Life Campaign. This campaign is set to launch on September 1, 2004, and although the campaign runs throughout the year, we are planning special events throughout the Pediatric Cancer Awareness Month of September. What is Lunch for Life? Lunch for Life gives kids an opportunity to help other kids just like themselves. These are kids who love to play outside, swim, fly kites, and play games, but because their young lives have been turned upside down by the words "You have cancer," they no longer can enjoy the activities of a "normal" child. Their lives are filled with doctor visits, injections, chemotherapy, radiotherapy, long days of hospitalization and countless hours of pain and, unfortunately, suffering. Until a cure is found, this scene will continue ...