AllDocs -> cancers

1 The transcription factor EGR-1 promotes apoptosis of neuroblastoma cells. Short title: Role of EGR-1 in neuroblastoma cell death. Miguel Pignatelli *‡ , Rosario Luna-Medina *‡ , Arturo Pérez-Rendón * , Angel Santos † & Ana Perez-Castillo * ‡ Both authors contributed equally to this paper * .- Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas- Universidad Autónoma, 28029-Madrid. † .- Departamento de Bioquímica y Biología Molecular, Facultad de.Medicina, Universidad Complutense, Madrid. Requests for reprints: - Ana Perez-Castillo. Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas-Universidad Autónoma, 28029-Madrid. Spain. Phone: 34-91-5854625, Fax: 34-91-5854587, E-mail: aperez@iib.uam.es . Or ...

1 DIAGNOSTIC IMAGING IN NEUROBLASTOMA Hans Ringertz, M.D., Ph.D. INTRODUCTION Neuroblastoma is a biologically very special neoplasm that is unique to child- hood. As can be seen from Table 1 it is the fourth most common paediatric malignancy in the Nordic countries and constitutes around 6% of all malignancies in children. Neoplasm in patients below 16 years of age in the Nordic countries during the years 1985 and 2001 in order of frequency No. of new cases % 1 Leukaemia 3249 34 2 Central nervous system and intracranial and intraspinal neoplasm 2596 27 3 Lymphomas and other reticuloendothelial neoplasm 1116 11 4 Sympathetic nervous system tumours 595 6 5 Renal tumours 556 6 6 Soft tissue sarcomas 540 5 7 Malignant bone tumours 297 3 8 Germ-cell, trophoblastic ...

[CANCER RESEARCH 63, 5266 –5273, September 1, 2003] Genome-wide Array CGH Analysis of Murine Neuroblastoma Reveals Distinct Genomic Aberrations which Parallel those in Human Tumors 1,2 Christopher S. Hackett, 3 J. Graeme Hodgson, 2 Mark E. Law, Jane Fridlyand, Kazutoyo Osoegawa, Pieter J. de Jong, Norma J. Nowak, Daniel Pinkel, Donna G. Albertson, Ajay Jain, Robert Jenkins, Joe W. Gray, and William A. Weiss 4 Departments of Neurology [C. S. H., W. A. W.], Pediatrics [C. S. H., W. A. W.], Neurological Surgery [C. S. H., W. A. W.], Laboratory Medicine [J. G. H., D. P., A. J., J. W. G., W. A. W.], Cancer Center [J. G. H., J. F., D. P., D. G. A., A. J., W. A. W.], and Cancer Research Institute [J. F., D. G. A., A. J., J. W. G.], University of California, San Francisco, California 94143-0114; Department of Laboratory Medicine and Pathology, Mayo ...

Journal of Clinical Epidemiology 54 (2001) 702–709 0895-4356/01/$ – see front matter © 2001 Elsevier Science Inc. All rights reserved. PII: S 0 8 9 5 - 4 3 5 6 ( 0 0 ) 0 0 3 3 9 - 5 Risk factors for neuroblastoma at different stages of disease. Results from a population-based case-control study in Germany Joachim Schüz*, Uwe Kaletsch, Rolf Meinert, Peter Kaatsch, Claudia Spix, Jörg Michaelis Institut für Medizinische Statistik und Dokumentation der Johannes Gutenberg-Universität, Mainz, D-55101 Mainz, Germany Received 10 December 1999; received in revised form 15 September 2000; accepted 21 September 2000 Abstract Neuroblastoma is one of the childhood cancers included in two recent population-based case-control studies in West Germany. Alto- gether, 183 children under the age of 8 with neuroblastoma diagnosed in 1988–1994 and 1785 control children sampled from population registration ...

I80 EPIDEMIOLOGY IN THE GERMAN NEUROBLASTOMA SCREENING PROJECT C. Spix, Dt. Kinderkrebsregister, Joh.Gutenberg Universität (Mainz) In Germany Neuroblastoma is the most frequent extracranial solid childhood tumor. Its properties made it seem an ideal candidate for screening. The German childhood cancer registry was involved in the project as epidemiological consultant and to ensure ascertainment of cases and deaths. A complete registry, which determines the incidence, age at diagnosis, stage distribution, and mortality in all relevant groups (control area and study area, participants and non participants) independently of the study and beyond the field phase of the study is an optimal basis for the evaluation of a screening study. Ascertainment was achieved by regular stochastic record linkage of encrypted personal identifier data from all 1.5 million study participants with the registry data at the ...

Last Revised: 3/5/2003 UM Cancer Center Patient Education Document #0007 Mar03 Ed. Online version: http://www.cancer.med.umich.edu/learn/percpathways.htm University of Michigan Comprehensive Cancer Center Patient Education Resource Center (PERC) INFORMATION GUIDE Childhood Cancer: Neuroblastoma The purpose of this information guide is to help parents of children newly diagnosed with Neuroblastoma find sources of information and support. This list is not meant to be comprehensive, but rather to provide starting points for information seeking. The materials can be found at the Patient Education Resource Center at the University of Michigan Comprehensive Cancer Center in room B1-361. Article Available in the Patient Education Resource Center level B1 room 361 Reynolds, Patrick C. Treating High-Risk Neuroblastoma at the ...

Biochem. J. (1996) 318, 1033–1039 (Printed in Great Britain) 1033 Agonist regulation of adenylate cyclase activity in neuroblastoma iglioma hybrid NG108-15 cells transfected to co-express adenylate cyclase type II and the ß 2 -adrenoceptor Evidence that adenylate cyclase is the limiting component for receptor-mediated stimulation of adenylate cyclase activity David J. M AC EWAN, Gun-Do KIM and Graeme MILLIGAN* Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K. Stable cell lines, derived from NG108-15 cells and transfected to express both the ß#-adrenoceptor and adenylate cyclase type II, were produced and examined. The absence of adenylate cyclase type II in the parental cells and its presence in these clones was ...

Clinical Categories of Neuroblastoma Are Associated with Different Patterns of Loss of Heterozygosity on Chromosome Arm 1p Jaume Mora,* Nai-Kong V. Cheung,* Brian H. Kushner,* Michael P. LaQuaglia, ‡ Kim Kramer,* Melissa Fazzari, † Glenn Heller, † Lishi Chen, § and William L. Gerald § From the Departments of Pediatrics, * Biostatistics, † Pediatric Surgery, ‡ and Pathology, § Memorial Sloan-Kettering Cancer Center, New York, New York Deletion of the short arm of chromosome 1 is fre- quently observed in neuroblastoma (NB). We per- formed loss of heterozygosity (LOH) analysis of 120 well characterized NB to better define specific regions of 1p loss and any association with clinical and bio- logical prognostic features (DNA index , MYCN ...

Abstract. Neuroblastoma (NB), one of the most common solid tumors among children, is histologically classified by the degree of maturation. To elucidate the mechanisms underlying its maturational sequence, we analyzed gene- expression profiles of 14 NB tumors on cDNA microarrays consisting of 23,040 genes. Computational analysis identified 78 genes whose expression levels were significantly different between differentiating NB tumors and poorly differentiated NB tumors. This group included genes associated with cell maturation and apoptosis. Among them we identified 15 that were up-regulated in Stage IV NB tumors; these included genes encoding cell adhesion molecules and cytoskeleton proteins. The set of genes we report here should contribute to a better understanding of NB tumor maturation and could lead to development of new therapeutic strategies. Introduction NB is the second most ...

P 100 IP3-MEDIATED CA2+ SIGNALS IN HUMAN NEUROBLASTOMA SH-SY5Y CELLS OVEREXPRESSING TYPE 3 IP3-RECEPTOR K. Van Acker, P. De Smet, A. Neyrinck, J.B. Parys, H. De Smedt, L. Missiaen AND G. Callewaert Laboratorium voor Fysiologie, K.U. Leuven, Campus Gasthuisberg, Herestraat 49, B-3000 Leuven IP3 receptors are ligand-gated channels that mediate Ca2+ release from ER in response to the second messenger IP3. Three isoforms of the receptor have been described and there is substantial evidence that the different isoforms display functional differences1. In human neuroblastoma SH- SY5Y cells type 1 IP3R is predominantly (>99%) expressed2. Here, we stably transfected SH- SY5Y cells with IP3R type 3 rat cDNA and studied the consequences on IP3-mediated Ca2+ release evoked by the muscarinic agonist carbachol (CCh). Immunocytochemistry experiments showed a homogeneous cytoplasmic distribution of type 3 and type 1 IP3 ...

Calcium Protects Differentiating Neuroblastoma cells During 50 Hz Electromagnetic Radiation R. Tonini, M.D. Baroni E. Masala, M. Micheletti + , A. Ferroni, and M. Mazzanti # . Dipartimento di Fisiologia e Biochimica Generali, I a Università di Milano, I-20133 Milano, Italy. + DIBIT, HSR, I-20100 Milano, Italy. # Dipartimento di Biologia Cellulare e dello Sviluppo, Università “La Sapienza”, I-00185 Roma, Italy. Running title: Magnetic field modulates NG 108-15 cells differentiation Keywords: extremely low frequency electromagnetic field (ELF-EMF), neuroblastomaXglioma cells (NG 108-15), BT2-cAMP induced differentiation, resting potential modulation, Calcium current. Corresponding author: Prof. Michele Mazzanti Dipartimento di Biologia Cellulare e dello Sviluppo Università "La Sapienza" P.le Aldo Moro 5, I-00185, ...

G007 PPAR : A regulator of diverse cellular responses in childhood neuroblastoma 1 V.C. Emmans, 1 H.A. Rodway, 2 A. Hunt and 1 K.A. Lillycrop School of Biological Sciences, University of Southampton Department of Child Health, Southampton General Hospital Neuroblastoma is an embryonal cancer of the sympathetic nervous system that arises from the neural crest and is the most common extra- cranial childhood tumour. However, uniquely some neuroblastomas spontaneously regress, which has prompted a search for agents, which can mimic this natural phenomenon. We have shown that ligands of the nuclear hormone receptor, peroxisome proliferators activated receptor gamma (PPAR ) inhibit the growth of neuroblastoma cells in vitro . However, the concentration of the natural PPAR ligand, 15-deoxy? 12,14 ...

66 0 20 40 60 80 100 120 140 160 180 200 220 Months Survival probabilities (%) 100 80 60 40 20 0 <1 y. (n=941) 1 y. (n=543) 2-14 y. (n=981) Age-standardized annual incidence rates (Germany: 1980-2001) Age- and sex-specific incidence rates (Germany: 1997-2001) Selected characteristics (Germany: 1997-2001) 11. Neuroblastoma Total number of cases in the registry (1980-2001): 2593 3-, 5-, and 10-year survival probabilities (Kaplan-Meier estimates): 70%, 65%, and 63% Survival probabilities by age-groups (Germany: 1980-2001) 0 2 4 6 8 10 <1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Girls Boys Incidence rates per 100,000 Age 0.0 0.4 0.8 1.2 1.6 2.0 1980 1983 1986 1989 1992 ...

The Notch signaling cascade in neuroblastoma : role of the basic helix- loop-helix proteins HASH-1 and HES-1. Håkan Axelson Department of Laboratory Medicine, Division of Molecular Medicine, Lund University, University Hospital MAS, S-205 02 Malmö, Sweden. Keywords: neuroblastoma , basic helix-loop-helix, MASH-1, HASH-1, HES-1, Notch-1, differentiation § To whom requests for reprints should be addressed: Håkan Axelson, Department of Laboratory Medicine, Division of Molecular Medicine, Lund University, University Hospital MAS, Entrance 78, S-205 02 Malmö. Phone: +46-40337621; Fax: +46-40337322; E-mail: hakan.axelson@molmed.mas.lu.se Page 2 Abstract Neuroblastoma is a childhood tumor originating from cells of the developing sympathetic nervous system. The disease exhibits a remarkable phenotypic diversity reflected in the ...

1 Cancer Letters, Special Issue 2003: “Neural development and Cancer” (Mini-review) Comprehensive genomics linking between neural development and cancer: Neuroblastoma as a model Akira Nakagawara*, Miki Ohira Division of Biochemistry, Chiba Cancer Center Research Institute, 666-2 Nitona, Chuoh-ku, Chiba 260-8717, Japan * Corresponding author. Tel: +81-43-264-5431; Fax: +81-43-265-4459. E-mail address: akiranak@chiba-ccri.chuo.chiba.jp (A. Nakagawara) Page 2 2 Abstract Cancer cells are derived from their precursor cells, which normally develop to the matured cells to form individual organs. Neuroblastoma , one of the most common pediatric solid tumors, originates from possible cancer stem cells derived from the neural crest. During the development, neural crest cells segregate into several lineages such as sensory, enteric and ...

· 853 · Zhang HY et al / Acta Pharmacol Sin 2003 Sep; 24 (9): 853-858 1 Project supported by the National Natural Science Foundation of China, No 301230050. 3 Correspondence to Prof TANG Xi-Can. Fax 86-21-5080-7088. E-mail xctang@mail.shcnc.ac.cn; and Prof Stephen BRIMIJOIN. Fax 507-284-9111, E-mail brimijoi@mayo.edu Received 2003-05-26 Accepted 2003-07-14 ©2003, Acta Pharmacologica Sinica Chinese Pharmacological Society Shanghai Institute of Materia Medica Chinese Academy of Sciences http://www.ChinaPhar.com Apoptosis induced by ß -amyloid 25-35 in acetylcholinesterase- overexpressing neuroblastoma cells 1 ZHANG Hai-Yan, Stephen BRIMIJOIN 2,3 , TANG Xi-Can 3 State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, ...

PPARs EXPRESSION IN HUMAN NEUROBLASTOMA CELL LINE. PPAR-LIGANDS INDUCE NEURONAL DIFFERENTIATION IN HUMAN NEUROBLASTOMA CELLS. B. D’Angelo, M.A. D’Amico M., Benedetti E., Cristiano L., Cerù M.P. and A. Cimini Dipartimento di Biologia di Base e Applicata, Università di L’Aquila PPARs belong to the steroid/thyroid/retinoid /vitamin D3 nuclear receptor superfamily and play fundamental roles in energy homeostasis by mediating FA effects on the expression of genes involved in lipid metabolism. The panel of genes regulated by PPARs is mainly involved in lipid metabolism, but genes regulating cell cycle and differentiation have also been recently recognized as PPAR target genes. The PPAR family comprises three closely related gene products, PPAR a , ß / d and ? with different tissue distribution, developmental expression and ligand specificity. Fatty acids, represent their natural ligands. Recently, ...

[CANCER RESEARCH 63, 1520 –1526, April 1, 2003] Antagonism of Buthionine Sulfoximine Cytotoxicity for Human Neuroblastoma Cell Lines by Hypoxia Is Reversed by the Bioreductive Agent Tirapazamine 1 Bo Yang, Nino Keshelava, Clarke P. Anderson, and C. Patrick Reynolds 2 Division of Hematology-Oncology, Children’s Hospital Los Angeles and Departments of Pediatrics [C. P. A., C. P. R.] and Pathology [C. P. R.], University of Southern California, Keck School of Medicine, Los Angeles, California 90027 ABSTRACT Relapse of neuroblastoma (NB) commonly occurs in hypoxic tissues. Buthionine sulfoximine (BSO), an inhibitor of glutathione (GSH) synthe- sis, is cytotoxic for NB cell lines in atmospheric oxygen (20% O 2 ). Tira- pazamine (TPZ) is a bioreductive agent that forms a toxic-free radical in hypoxia. We determined in four NB cell lines ...

Calcium Waves in Differentiated Neuroblastoma Cells Catherine Lloyd Table of Contents Model Structure .. .. 1 Download This Model . 2 Model Structure Intracellular calcium dynamics are frequently the subject of theoretical mathematical model s (De Young and Keizer, 199 2 [IP3_Ca2+_model_1992_doc.html], Li and Rinzel, 1994 [li_model_1994_doc.html], Keizer and Levine , 1996 [keizer_model_1996_doc.html], Jafri-Rice-Winslow, 1998 [JRW_model_1998_doc.html], and Sny der et al., 2000 [snyder_model_2000_doc.html] are just a few examples of calcium dynamic models that have been coded up into CellML). The physical and chemical laws of calcium waves and oscillations can be expressed in terms of differential equations describing re- action kinetics, fluxes through membranes, and diffusion. In this study, Charles C. Fink et al . produce an image-based ...

NBL 07/09/01 Page 1 of 2 Study Summary ANBL00P2 Perinatal Neuroblastoma : Expectant Observation: A COG Pilot Study This study evaluates treatment for infants with a cyst or small mass in the adrenal gland (a gland found over each kidney that secretes hormones). In many cases such a mass is a tumor called neuroblastoma , although it could also represent a lung malformation called pulmonary sequestration, or a blood clot in the adrenal gland, referred to as adrenal hemorrhage. The current standard treatment for neuroblastoma is surgical removal of the tumor. We have learned a great deal about this tumor recently. One of the key findings is that it behaves quite differently in infants compared to older children. Several recent studies have suggested that anywhere between 33 and 100% of these small adrenal tumors in young infants spontaneously resolve (disappear on ...