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Survival of children with neuroblastoma : time trends and regional di?erences in Europe, 1978±1992 C. Spix a, *, T. Aareleid b,c , C. Stiller d , C. Magnani e , P. Kaatsch a , J. Michaelis a a Institute for Medical Statistics and Documentation, German Childhood Cancer Registry, University Mainz, 55101 Mainz, Germany b Department of Epidemiology and Biostatistics, Institute of Experimental and Clinical Medicine, Tallinn, Estonia c Estonian Cancer Registry, Estonian Cancer Centre, Tallinn, Estonia d National Registry of Childhood Tumours, Childhood Cancer Research Group, Oxford, UK e Childhood Cancer Registry of Piedmont. Regional Centre for Cancer Epidemiology and Prevention in Piedmont. Turin, Italy Received 27 September 2000; accepted 27 December 2000 Abstract ...

Journal of Clinical Epidemiology 54 (2001) 702–709 0895-4356/01/$ – see front matter © 2001 Elsevier Science Inc. All rights reserved. PII: S 0 8 9 5 - 4 3 5 6 ( 0 0 ) 0 0 3 3 9 - 5 Risk factors for neuroblastoma at different stages of disease. Results from a population-based case-control study in Germany Joachim Schüz*, Uwe Kaletsch, Rolf Meinert, Peter Kaatsch, Claudia Spix, Jörg Michaelis Institut für Medizinische Statistik und Dokumentation der Johannes Gutenberg-Universität, Mainz, D-55101 Mainz, Germany Received 10 December 1999; received in revised form 15 September 2000; accepted 21 September 2000 Abstract Neuroblastoma is one of the childhood cancers included in two recent population-based case-control studies in West Germany. Alto- gether, 183 children under the age of 8 with neuroblastoma diagnosed in 1988–1994 and 1785 control children sampled from population registration ...

Summary Iodine-123-MIBG Scintigraphy in Neuroblastoma ; Relationship between the Intensity of Uptake and Tumor Characteristics Chio O KUYAMA , Yo U SHIJIMA , Kaori W ATANABE , Hiroki S UGIHARA and Tsunehiko N ISHIMURA Department of Radiology, Kyoto Prefectural University of Medicine Iodine-123-MIBG ( 123 I-MIBG) scintigraphy were performed for 23 patients with neuroblastoma at diag- nosis. The intensity of MIBG activity in the primary tumor was evaluated visually (grade 3; intense uptake ~grade 0; no definite uptake), and its relationship to the size, degree of tumor spread, urinary catechol- amine metabolites (VMA, HVA), and histological types were investigated. The results of 123 I-MIBG uptake grade were as fol- lows: grade 3; 44% (10/23), grade 2; 30% (7/23), ...

neuroblastoma F-18 Genetics groups of Neuroblastoma Patients defined by molecular and conventional cytogenetics Leticia Glaser-Gabay 1, Marta Jeison 1, Jacques Mardouk 1, Drorit Luria2, Jerry Stein2, Yacov Goshen2, Rina Zaizov 2, Isaac Yaniv 2, Batia Stark 1 2 1Cancer Cytogenetic Laboratory, 2Pediatric Oncology /Hematology Center, Schneider Children?s Medical Center of Israel, Sackler School of Medicine, Tel-Aviv University, Israel. Neuroblastoma (NBL) is one of the most common solid tumors in children expressing a wide clinical spectrum from spontaneous regression or differentiation to highly metastatic fatal disease. In order to assess the genetic heterogeneity of this disease and to correlate it to the clinical characteristics, we have analyzed 59 NBL patients using conventional and molecular cytogenetic techniques and ploidy. Fluorescence-in- situ-Hybridization (FISH) probes included: ...

NEUROBLASTOMA 2002 American Cancer Society Neuroblastoma – 1/17/01 What Is Cancer? Cancer is a group of many related diseases. All forms of cancer cause cells in the body to change and grow in an abnormal way. Normal body cells divide and grow in an orderly fashion. But cancer cells divide and grow out of control. This out-of-control growth damages normal body tissues and disrupts the ability of organs to function as they should. During the early years of a person's life, normal cells divide more rapidly until adult size is reached. After that, normal cells of most tissues divide only to replace worn-out tissue and to repair injuries. Cancer cells, however, continue to grow. Often the cells form tumors (lumps) that compress, invade, and destroy normal tissue. Unless the cancer is treated, the tumors can grow and spread. If cells break away from such a tumor, they ...

Biochem. J. (2001) 360, 569–577 (Printed in Great Britain) 569 RB2/p130 ectopic gene expression in neuroblastoma stem cells : evidence of cell-fate restriction and induction of differentiation Francesco P. JORI*, Umberto GALDERISI † 1 , Elena PIEGARI †, Gianfranco PELUSO‡, Marilena CIPOLLARO†, Antonio CASCINO†, Antonio GIORDANO § and Mariarosa A. B. MELONE* *Department of Neurological Sciences, Second University of Naples, Via Pansini 5, 80100 Naples, Italy, †Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy, ‡Institute of Biochemistry of Proteins and Enzymology, C. N. R., Arco Felice, Naples, Italy, and §Department of Pathology, Anatomy and Cell Biology, Jefferson Medical College, and Sbarro Institute for Cancer Research and Molecular Medicine, 1020 Locust ...

Erythropoietin (Epo), the principal regulator of erythro- poiesis, is a glycoprotein hormone produced primarily in the adult kidney and fetal liver (for review see Jelkmann, 1992; Fisher, 1997). The levels of Epo in the organism are regulated by oxygen tension since hypoxic conditions increase Epo gene expression. Erythroid precursor cells, which occur in the adult bone marrow and fetal liver, are known to express the Epo receptor (EpoR) on the cell surface. Binding of Epo to its receptor induces differentiation and proliferation of these precursor cells (for review see Damen & Krystal, 1996). Recently, EpoR expression has also been observed in the central nervous system and in neuronal cell lines. In particular, a functional EpoR was detected in PC12 and SN6 cells, two cell lines with neuronal characteristics (Masuda et al. 1993). Specific Epo binding sites have been localized ...

Calcium Protects Differentiating Neuroblastoma cells During 50 Hz Electromagnetic Radiation R. Tonini, M.D. Baroni E. Masala, M. Micheletti + , A. Ferroni, and M. Mazzanti # . Dipartimento di Fisiologia e Biochimica Generali, I a Università di Milano, I-20133 Milano, Italy. + DIBIT, HSR, I-20100 Milano, Italy. # Dipartimento di Biologia Cellulare e dello Sviluppo, Università “La Sapienza”, I-00185 Roma, Italy. Running title: Magnetic field modulates NG 108-15 cells differentiation Keywords: extremely low frequency electromagnetic field (ELF-EMF), neuroblastomaXglioma cells (NG 108-15), BT2-cAMP induced differentiation, resting potential modulation, Calcium current. Corresponding author: Prof. Michele Mazzanti Dipartimento di Biologia Cellulare e dello Sviluppo Università "La Sapienza" P.le Aldo Moro 5, I-00185, ...

Stimulation of using a Human Neuroblastoma Cells Planar Microelectrode Array Page 2 A thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the University of Glasgow. October 2000 Carolyn Audrey McEwan Centre for Cell Engineering University of Glasgow Stimulation of using a Human Neuroblastoma Cells Planar Microelectrode Array

24 p53 response and function in neuroblastoma cell lines. B. Nelson, M. Elliott, H.P. McDowell, M.R.White University of Liverpool, SOBS, Life Sciences Building, Crown Street, Liverpool, L69 7ZB. INTRODUCTION Neuroblastoma is the most common solid childhood cancer and the prognosis for this condition remains poor. p53 mutations are rare in neuroblastoma , suggesting possible defects downstream of p53 in the apoptotic pathway. METHODS Using neuroblastoma cell lines we have quantified p53 expression and its downstream targets by Western Blotting, after treatment with DNA damaging agents. The transcriptional activity of p53 was determined using a luciferase reporter gene. Real time fluorescence confocal microscopy of living cells and flow cytometry were used to characterise the timing and nature of cell death in response to DNA damaging agents. RESULTS 1. The ...

CMLS, Cell. Mol. Life Sci. 57 (2000) 514 – 520 1420-682X/00/030514-07 $ 1.50 + 0.20/0 © Birkha¨user Verlag, Basel, 2000 Research Article Resting potential of excitable neuroblastoma cells in weak magnetic fields H. Sonnier a , O. V. Kolomytkin b and A. A. Marino a, * a Department of Orthopedic Surgery, LSU Medical Center in Shreveport, P.O. Box 33932, Shreveport (Louisiana 71130-3932, USA), Fax + 1 318 675 6186, e-mail: amarino@lsumc.edu b Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino 142292 (Russia) Received 15 September 1999; received after revision 11 November 1999; accepted 5 January 2000 Abstract. The mechanism by which static and low-fre- 5-s exposure immediately followed by a 5-s control period. In each experiment, the field had no discernible quency magnetic fields ...

1 Expression of Recombinant Prion Protein in Mouse Neuroblastoma Cell Lines Sebastian Mathews, Foo Cheung, Ph.D, Clarence J. Gibbs, MD ABSTRACT : In an attempt to eventually determine the best way to inhibit the expression of abnormal Prion protein, the causative agent of Transmissible Spongiform Encephalopathies, an experiment was conducted in which genomic DNA was extracted from the white blood cells of three patients, each carrying different genetic mutations for the familial Prion protein disease known as Creutzfeldt-Jakob syndrome. Once extracted, the Prion protein coding region of the DNA was PCR amplified, ligated into an Eukaryotic expression vector (pCR 3.1), and transfected into mouse neuroblastoma cell lines for transient protein expression. Production of recombinant Prion protein was successful. It was found that the proteins derived from the three mutations ...

EXPERIMENTAL and MOLECULAR MEDICINE, Vol. 33, No. 1, 37-45, March 2001 Adaptation of cAMP signaling system in SH-SY5Y neuroblastoma cells following expression of a constitutively active stimulatory G protein alpha, Q227L Gs a Ik-Soon Jang 1 and Yong-Sung Juhnn 1,2 1 Department of Biochemistry and Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Korea 2 Corresponding author: Tel, +82-2-740-8247; Fax, +82-2-744-4534; E-mail, juhnn@snu.ac.kr Accepted 23 March, 2000 Abbreviations: AC, adenylate cyclase; ACI, AC isoform I; dbcAMP, dibutyryl cAMP; GAPDH, glyceraldehyde-3-phosphate dehydroge- nase; Gs a , alpha subunit of stimulatory G protein; PDE, cyclic nucleotide phosphodiesterase; PKA, cAMP-dependent protein kinase; RT-PCR, reverse transcription-polymerase chain ...

Session: Screening evaluation, Sept. 13, 12:00 The German Neuroblastoma Screening Project – evaluation methods and preliminary results S PIX C, S CHILLING FH, M ICHAELIS J gsf / IMSO, Uni Mainz, spix@imso.uni-mainz.de Session: Screening evaluation, Sept. 13, 12:00 Neuroblastoma is the most frequent solid childhood tumor with a cumulative incidence of ca.15/100 000. As the frequency of high stages increases with age and the prognosis for the disseminated stage 4 is considerably worse than that for the localized stages, a benefit from early diagnosis seems likely. Since 1995 a project evaluating the effect of screening infants with a simple urine test at age 12 months is conducted in half of Germany, with the other half serving as a control. The project is scheduled to be evaluated after 2001. Earlier screening programmes (Japan, Canada) ...

Biochem. J. (1996) 318, 1033–1039 (Printed in Great Britain) 1033 Agonist regulation of adenylate cyclase activity in neuroblastoma iglioma hybrid NG108-15 cells transfected to co-express adenylate cyclase type II and the ß 2 -adrenoceptor Evidence that adenylate cyclase is the limiting component for receptor-mediated stimulation of adenylate cyclase activity David J. M AC EWAN, Gun-Do KIM and Graeme MILLIGAN* Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K. Stable cell lines, derived from NG108-15 cells and transfected to express both the ß#-adrenoceptor and adenylate cyclase type II, were produced and examined. The absence of adenylate cyclase type II in the parental cells and its presence in these clones was ...

Neuroblastoma Authors: Doctors Matthias Schell 1 and Christophe Bergeron Creation date: October 2003 Scientific Editor: Professor Thierry Philip 1 Centre Léon Bérard, Service de Pédiatrie, 69373 Lyon cedex 08, France. schell@lyon.fnclcc.fr Abstract Key-words Disease name and synonyms Definition Differential diagnosis Etiology Clinical description Outcome Diagnostic methods Epidemiology Treatment Unresolved questions References Abstract Neuroblastoma is a malignant tumour of neural crest cells which give rise to the sympathetic nervous ...

Detection of Amplification in Neuroblastoma Using Polymerase Chain Reaction and Its Impact on Survival A P RATAP , S S INHA , DK G UPTA Molecular biology has revolutionized the medicine and added a new dimension to our knowledge of disease processes, especially in the field of oncology. Proto oncogenes are proteins normally present in the genome and they may be converted to oncogenes by mutations, rearrangements, translocation and deletion. One such protooncogene is the N-myc proto-oncogene seen in neuroblastoma . Amplification of the N-myc oncogene identifies a group of patients who have a poor prognosis. At present, it is generally agreed that the amplification of N-myc is still the most important prognostic factor to be investigated in every patient with neuroblastoma because of it’s reliability as a prognostic indicator. There are many conventional ...

PINAL neuroblastoma is an extremely rare tumor in adults, with only four cases reported in the litera- ture. 1,4,9,18 The prognosis for patients with this tumor has been uniformly poor, with no long-term survival pre- viously reported. We describe a case of spinal neuroblas- toma in a 32-year-old woman who had presented with a history of retroperitoneal ganglioneuroma 11 years earlier. The patient has survived 10 more years with relatively sta- ble disease and the recent development of metastases. To our knowledge, this is the first report of malignant trans- formation of ganglioneuroma into neuroblastoma . Case Report History. In 1976, at 21 years of age, this 42-year-old woman presented with a retroperitoneal mass. The mass was removed at another institution and was diagnosed as ganglioneuroma. Further clinical and surgical details are not available. The surgical ...

NTRASELLAR neuronal neoplasms involving the pitu- itary gland are very uncommon. Li, et al ., 23 identified only six tumors with a ganglionic component in their collection of 2000 intrasellar neoplasms. Although clini- cally progressive, occasionally recurrent, and sometimes fatal, all of the tumors described were well-differentiated and histologically benign choristomas and hamartomas. All were composed of a mixture of mature neurons and glial stroma, occasionally accompanied by epithelial ade- noma cells. 1,4–6,9,11,12,18–20,22,23,31,34–36,38–42 In more than 50% of cases, hypothalamic neuronal cho- ristomas are associated with overt clinical endocrinopa- thies, most often precocious puberty and acromegaly. 1,6,8,9, 11,12,18–20,23,29,31,34,36,38–41,43,45 Until recently, 15 subpopulations of these tumors with an epithelial component ...

D ATA S HEET Rev 030502D Neuroblastoma Marker Ab-1 (Clone NB84a) Mouse Monoclonal Antibody Cat. #MS-492-S0, -S1, or -S (0.1ml, 0.5ml, or 1.0ml Supernatant) Cat. #MS-492-R7 (7.0ml) (Ready-to-Use for Immunohistochemical Staining) Cat. #MS-492-PCS (5 Slides) (Positive Control for Histology) #&&( ÃXrvtu?rÃ9? ÃA?r?Ã86ÃÃ(#$"(ÃVT6Ã Ury)Ã ' Ã'!' %!'ÃÃAh?)Ã $ Ã(( !'!%Ã @?hvy)Ãyhi?v?v?5yhi?v?v? pÃÃXri?v?r)Ã yhi?v?v? p Comments: Ab-1 recongizes a protein of 57kDa which is found in a wide range of normal tissues, including most epithelia and endothelial cells. Ab-1 is useful in identification of pediatric, small round blue cell tumors. Approximately 90% of neuroblastomas are stained by this antibody whereas all other tumor types such as leukemia, rhabdomyosarcomas, and Wilm’s tumors are ...

Last Revised: 3/5/2003 UM Cancer Center Patient Education Document #0007 Mar03 Ed. Online version: http://www.cancer.med.umich.edu/learn/percpathways.htm University of Michigan Comprehensive Cancer Center Patient Education Resource Center (PERC) INFORMATION GUIDE Childhood Cancer: Neuroblastoma The purpose of this information guide is to help parents of children newly diagnosed with Neuroblastoma find sources of information and support. This list is not meant to be comprehensive, but rather to provide starting points for information seeking. The materials can be found at the Patient Education Resource Center at the University of Michigan Comprehensive Cancer Center in room B1-361. Article Available in the Patient Education Resource Center level B1 room 361 Reynolds, Patrick C. Treating High-Risk Neuroblastoma at the ...