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1 Braz J Med Biol Res 2006 Online Ahead of Print Dexamethasone blocks SK-N-SH cell migration Brazilian Journal of Medical and Biological Research 2006 Online Ahead of Print ISSN 0100-879X Dexamethasone blocks the migration of the human neuroblastoma cell line SK-N-SH 1 Escola Superior em Ciências da Saúde, Brasília, DF, Brasil 2 Department of Endocrinology, Center of Endocrinological Oncology, University of Milano, Milano, Italy 3 Department of Endocrinology and Diabetology, Wroclaw Medical University, Wroclaw, Poland L.A. Casulari 1 , D. Dondi 2 , R. Maggi 2 , M. Demissie 3 , M. Piccolella 2 and F. Piva 2 Abstract Glucocorticoids (Gc) influence the differentiation of neural crest- derived cells such as those composing sympathoadrenal ...

Research Article (R)-Roscovitine (CYC202, Seliciclib) sensitizes SH-SY5Y neuroblastoma cells to nutlin-3-induced apoptosis Judit Ribas a,b , Jacint Boix b , Laurent Meijer a, ? a CNRS, Cell Cycle Group, Station Biologique, B.P. 74, 29682 Roscoff cedex, Bretagne, France b University of Lleida, School of Medicine, Molecular Pharmacology Group, C/Montserrat Roig 2, 25008 Lleida, Catalunya, Spain A R T I C L E I N F O R M A T I O N A B S T R A C T Article Chronology: Received 31 October 2005 Revised version received 8 March 2006 Accepted 6 April 2006 Available online 13 May 2006 In this study, we have analyzed the consequences, on several neuroblastoma cell lines, of combined treatments with (R)-roscovitine (CYC202, Seliciclib), a CDK inhibitory drug, and nutlin-3, a p53 activating drug. ...

Thiele CJ. Neuroblastoma : In (Ed.) Masters, J. Human Cell Culture. Lancaster, UK: Kluwer Academic Publishers. 1998, Vol 1, p 21-53 Neuroblastoma Cell Lines Carol J. Thiele Cell & Molecular Biology Section, Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bld. 10 Rm 13N240, 10 Center Dr. MSC1928, Bethesda, MD. 20892-1928; fax 301-402-0575; thielec@pbmac.nci.nih.gov Page 2 2 Historical Introduction Neuroblastoma accounts for approximately 9% of all childhood cancers, occurring once out of 8,000 live births. This results in an annual incidence of approximately 1 in 10 5 children less than 15 years of age world-wide (1) . The median age at diagnosis is approximately 22 months with over one-third diagnosed at less than 1 year of age and over 88% diagnosed by the age of 5. Some studies ...

Mechanisms of bone invasion and metastasis in human neuroblastoma Yasuyoshi Sohara a , Hiroyuki Shimada b , Yves A. DeClerck a,c, * a Division of Hematology–Oncology, Department of Pediatrics, Childrens Hospital Los Angeles, University of Southern California Keck School of Medicine, Los Angeles, CA, USA b Department of Pathology, Childrens Hospital Los Angeles, University of Southern California Keck School of Medicine, Los Angeles, CA, USA c Department of Biochemistry and Molecular Biology, Childrens Hospital Los Angeles, University of Southern California Keck School of Medicine, Los Angeles, CA, USA Received 2 December 2004; accepted 12 January 2005 Abstract Bone is the second most common site of metastasis in neuroblastoma . Over the last several years, our understanding of the mechanism of bone metastasis in ...

JaumaI a/Neurochemistry Raven Press, Ltd., New York @ 1989 International Society for Neurochemistry Uptake of Exogenous Phosphatidylserineby Human Neuroblastoma Cells Stimulates the Incorporation of [methyl-14C]Cholineinto Phosphatidylcholine B. E. Slack, M. Liscovitch, J. K. Blusztajn, and R. J. Wurtman Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, U.S.A. Abstract: The phosphatidylserine (PtdSer) content of human cholinergic neuroblastoma (LA-N-2) cells was manipulated by exposing the cells to exogenous PtdSer, and the effectson phospholipid content, membrane composition, and incor- poration of choline into phosphatidylcholine (PtdCho) were investigated. The presence of liposomes containing PtdSer (l0-130 p.M)in the medium caused time- and concentration- dependent increases in the PtdSer content of the cells, and ...

0502 Differentiation of Human Neuroblastoma SH-SY5Y Cells with the Neuroprotective Peptide PACAP TK Monaghan 1 , R Plevin 2 , EM Lutz 1 1 Dept. of Bioscience, University of Strathclyde, Royal College Building, 204 George St., Glasgow, G1 1XW, UK 2 Dept. of Physiology and Pharmacology, University of Strathclyde, SIBS, Taylor St., Glasgow, G4 0NG, UK Pituitary adenylate cyclase-activating polypeptide (PACAP) is a 38 amino acid neuropeptide with numerous functions throughout the nervous system. PACAP and its receptors are expressed early in the developing nervous system where they are thought to regulate neuronal prolifera- tion and differentiation. In addition, PACAP expression is up-regulated in the mature nervous system after neuronal trauma (e.g. physical injury or ischemia) where PACAP is believed ...

J. Biosci., Vol. 9, Numbers 3 & 4, December 1985, pp. 145-157. © Printed in India. Supra-molecular organization of adenylate cyclase in a neuroblastoma × glioma hybrid SHAIL ?. SHARMA Department of Biochemistry, All India Institute of Medical Sciences, New Delhi 110029, India MS received 2 March 1985 Abstract. Studies on the thermal inactivation of adenylate cyclase from neuroblastoma × glioma hybrid cells have been carried out. Inactivation curves show marked deviation from first-order kinetics, and as a first approximation can be adequately described as a sum of two negative exponentials. Half-lives ofthe rapidly decaying component have been estimated to be 5, 3·4,1·2 and 0·5 min at 37, 40, 44 and 48°C, respectively. The corresponding values for the slow-decaying component are found to be 90, 30, 11 and 5 min. Plausible inactivation pathways responsible for multi-exponential decay ...

Abstract. Background: The dietary carotenoid zeaxanthin protects against age-related eye disease by preventing apoptosis in photoreceptor cells. This study examined the effect of zeaxanthin on neuroblastoma cells in which apoptosis can be induced with lipid peroxidation products. Since zeaxanthin can inhibit lipid peroxidation and ‚-carotene inhibits lipoxygenase (LOX) activity, it was of concern that zeaxanthin might inhibit apoptosis in these cancer cells. Materials and Methods: Apoptosis-resistant CHP100 neuroblastoma cells were treated with zeaxanthin. Apoptosis was assessed via an immunoassay for histone-associated DNA fragments and cytofluorimetric analysis of apoptotic body formation. The effect of zeaxanthin on the activity of two model LOXs and LOX-mediated lipid peroxidation in liposomes was assessed. Results: Zeaxanthin strongly induced apoptosis in ...

Data Sheet Neuroblastoma Marker FITC-Conjugated mouse monoclonal antibody NCL-NB84-FITC Intended Use FOR RESEARCH USE ONLY . Specificity Uncharacterised molecule of 57kD found in most normal human tissues, including most epithelial and endothelial cells. Clones NB84a Ig Classes IgG1 Antigen used Human neuroblastoma tissue. for immunisations Hybridoma partner Mouse myeloma (p3-NS1-Ag4-1). Preparation Purified mouse monoclonal antibody conjugated to fluorescein isothiocynate isomer 1, containing 15mM sodium azide. Volume as indicated on vial label. Recommendations on use Direct flow cytometry: Intracellular staining procedure (see overleaf). Positive Controls Direct flow cytometry - Kelly cell line. Storage and stability Store the conjugated antibody at 4ºC. The conjugate ...

Olfactory Neuroblastoma : MR Evaluation Cheng Li,I.2.3 David M. Yousem,I.2.3 Richard E. Hayden,2 and Richard L. Dotyl.2 PURPOSE: To evaluate MR in the diagnosis and staging of olfactory neuroblastoma . METHODS: Five patients with histologically proved olfactory neuroblastomas were studied by MR. Four also had CT scans. RESUL TS: The extent of disease delineated by MR agreed with surgical findings and surgical staging of the tumor in all five patients. All cases of olfactory neuroblastomas originated in the nasal cavity and involved the ethmoid sinuses. In three of the five cases, the tumors extended to the anterior skull base. In one case MR correctly suggested skull base involvement missed on CT. Lesions were isointense to hyperintense with muscle on Tl-weighted scans. Compared with fat on T2-weighted scans, olfactory neuroblastomas were variable in signal intensity. Enhancement was minimal to moderate in ...

BC Children’s Hospital Oncology/Hematology/BMT Program Page 1 of 2 Neuroblastoma Rev Apr 2003 Neuroblastoma • Tumor that can arise anywhere in the sympathetic nervous system which consists of: ~ the adrenal glands, located one on top of each kidney ~ the ganglia, collection of nerve cells that lie on both sides of the spine from the neck to the pelvis and along the abdominal aorta • Most neuroblastomas occur in the abdomen and are often quite large • Aggressive tumors that in 70% of cases have spread to the bones and bone marrow at the time of diagnosis • Most common childhood cancer after leukemia and brain tumors • Average age is two years. 75% of diagnoses are made in the under four year old age group • Most children under one year of age do well even when many organs are involved. Poor survival when metastatic in patients over one year of age Definition: Neuroblastoma ...

Texas Children’s Cancer Center and Hematology Service Neuroblastoma What is neuroblastoma ? Neuroblastoma is a type of cancer that arises from the autonomic nervous system in the body. The autonomic nervous system controls things like blood pressure in the arteries, movement within the intestines, sweating, and many other activities. Parts of the autonomic nervous system lie along the spine and in the adrenal glands above the kidneys. Neuroblastoma may start in any of these areas. Most often, it starts in the abdomen—either in the adrenal glands or around the spinal cord. Neuroblastoma can also start around the spinal cord in the chest, neck, or pelvis. It can spread to lymph nodes, liver, skin, bone marrow, bone, lungs and brain. Neuroblastoma is one of the most common kinds of cancer in children. It occurs most often in ba- bies and very young children. ...

C IGNA H EALTH C ARE C OVERAGE P OSITION Subject Stem-Cell Transplant for Neuroblastoma Revised Date 10/15/2005 Original Effective Date ..10/15/2004 Coverage Position Number . 0189 Table of Contents Related Coverage Positions Stem-Cell Transplant for Central Nervous System Tumors Coverage Position..1 General Background .1 Coding/Billing Information..7 References.8 INSTRUCTIONS FOR USE Coverage Positions are intended to supplement certain standard CIGNA HealthCare benefit plans. Please note, the terms of a participant’s particular benefit plan document [Group Service Agreement (GSA), Evidence of Coverage, Certificate of Coverage, Summary Plan Description (SPD) or similar plan document] may differ significantly from the ...

MEDIA RELEASE April, 2005 New development in fight against childhood cancer Researchers at the Flinders Medical Centre have discovered a way to ‘switch off’ drug resistant genes to increase the chances of survival among children diagnosed with blood- related cancers. The research team, lead by Dr Bryone Kuss from the Department of Haematology and Genetic Pathology, has explored the role of certain drug resistant genes in neuroblastoma tumour cells and is investigating new approaches for the treatment of both neuroblastoma and acute leukemia. “It is not uncommon for cancers to become resistant to chemotherapy or radiotherapy and ultimately lead to cancer relapse,” Dr Kuss said. The team’s aim is to understand the mechanism behind the resistance in the cancers, which predominately affect children from birth. Although cure of acute leukemia is achievable in approximately 80% ...

Educational Session Neuroblastoma ASCO 2005 ASCO High-Risk Neuroblastoma : Beyond Intensification to Novel Therapy Approaches to Improve Outcome Chair Katherine K. Matthay, MD Professor of Pediatrics Chief, Pediatric Hematology-Oncology University of California San Francisco School of Medicine San Francisco, CA 94143-0106 Speakers C. Patrick Reynolds, MD, PhD Professor of Pediatrics Director, Developmental Therapeutics Program USC-CHLA Institute for Pediatric Clinical Research Children’s Hospital Los Angeles University of Southern California Los Angeles, Ca 90027 Rogier Versteeg, MD Professor and Chair, Human Genetics Academic Medical Center University of Amsterdam P.O. Box 22700 1100 DE Amsterdam The Netherlands Page 2 Educational Session ...

Efficacy of Complete Resection for High-Risk Neuroblastoma : A Children’s Cancer Group Study By E. Stanton Adkins, Robert Sawin, Robert B. Gerbing, Wendy B. London, Katherine K. Matthay, and Gerald M. Haase Columbia, South Carolina; Seattle, Washington; Arcadia, California; Gainesville, Florida; San Francisco, California; and Denver, Colorado Background/Purpose: Previous reports indicate that com- plete resection of high-risk neuroblastoma improves out- come but may entail high surgical complication rates. The authors evaluated the effect of complete primary site resec- tion on event-free survival (EFS), overall survival (OS), and complication rates in patients entered on a high-risk neuro- blastoma treatment protocol. Methods: A total of 539 eligible patients with high-risk neu- roblastoma were entered on protocol CCG-3891. Patients were assigned randomly to continuation chemotherapy ...

Last Revised: 3/21/2005 UM Cancer Center Patient Education Document #0007 Mar04 Ed.5 Online version: http://www.cancer.med.umich.edu/learn/percpathways.htm University of Michigan Comprehensive Cancer Center Patient Education Resource Center (PERC) INFORMATION GUIDE Childhood Cancer: Neuroblastoma The purpose of this information guide is to help parents of children newly diagnosed with Neuroblastoma find sources of information and support. This list is not meant to be comprehensive, but rather to provide starting points for information seeking. The materials can be found at the Patient Education Resource Center at the University of Michigan Comprehensive Cancer Center in room B1-361. Article Available in the Patient Education Resource Center level B1 room 361 Reynolds, Patrick C. Treating High-Risk Neuroblastoma at the Turn ...

Reexpression of Vimentin in Differentiated Neuroblastoma Cells Enhances Elongation of Axonal Neurites Maya Dubey, 1 Sadaf Hoda, 1 Walter K.-H. Chan, 1 Aurea Pimenta, 2 Daniela D. Ortiz, 1 and Thomas B. Shea 1 * 1 Center for Cellular Neurobiology and Neurodegeneration Research, Department of Biological Sciences, University of Massachusetts Lowell, Lowell, Massachusetts 2 Department of Pharmacology, Vanderbilt University, Nashville, Tennessee Vimentin (Vm) is initially expressed by early neuronal precursors in situ and in culture. Vm is essential for neuritogenesis at least in culture and is gradually re- placed by neurofilaments (NFs) because of down- regulation of Vm expression. This period is accompanied by a slowing of axonal elongation. We examined whether ...

Neuroblastoma - Overview © copyright 2004 U-M Comprehensive Cancer Center The University of Michigan Health System web site does not provide specific medical advice and does not endorse any medical or professional service obtained through information provided on this site or any links to this site. Use of the UMHS web site does not replace medical consultation with a qualified health or medical professional to meet the health and medical needs of you or others. While the content of the UMHS web site is frequently updated, medical information changes rapidly and therefore, some information may be out of date, and/or contain inaccuracies or typographical errors. Neuroblastoma (noor-oh-blas-TOME-ah) is the second most common solid tumor in childhood after brain tumors. The median age of diagnosis is two years and 85 percent of children diagnosed are less than 5. This tumor can arise in many parts ...

Section 4 – Your Child’s Diagnosis – Neuroblastoma Page 1 of 3 Neuroblastoma What is a Neuroblastoma ? Neuroblastoma is a tumour that begins in nerve tissues in the neck, chest, abdomen, or pelvis. About 50% of neuroblastomas start in the tissues of the adrenal glands located just above the kidneys. Often this tumour has spread before it is diagnosed. The common sites are the lymph nodes, liver, bones, and bone marrow. Neuroblastoma occurs in early childhood with 2/3 of the children younger than 5 years of age when they are diagnosed. Some neuroblastomas in infants can spontaneously regress and your doctor will discuss this with you. What causes a neuroblastoma ? As with most cancers, the cause of neuroblastoma is unknown. What are the signs and symptoms? The symptoms will vary depending on where the tumour is located. The most common symptoms are ...

G007 PPAR : A regulator of diverse cellular responses in childhood neuroblastoma 1 V.C. Emmans, 1 H.A. Rodway, 2 A. Hunt and 1 K.A. Lillycrop School of Biological Sciences, University of Southampton Department of Child Health, Southampton General Hospital Neuroblastoma is an embryonal cancer of the sympathetic nervous system that arises from the neural crest and is the most common extra- cranial childhood tumour. However, uniquely some neuroblastomas spontaneously regress, which has prompted a search for agents, which can mimic this natural phenomenon. We have shown that ligands of the nuclear hormone receptor, peroxisome proliferators activated receptor gamma (PPAR ) inhibit the growth of neuroblastoma cells in vitro . However, the concentration of the natural PPAR ligand, 15-deoxy? 12,14 prostaglandin ...

Extending Positron Emission Tomography Scan Utility to High-Risk Neuroblastoma : Fluorine-18 Fluorodeoxyglucose Positron Emission Tomography as Sole Imaging Modality in Follow-Up of Patients By Brian H. Kushner, Henry W.D. Yeung, Steven M. Larson, Kim Kramer, and Nai-Kong V. Cheung Purpose: Although positron emission tomography (PET) with fluorine-18 fluorodeoxyglucose ( 18 F-FDG) has a major impact on the treatment of adult cancer, the reported experience with extracranial tumors of child- hood is limited. We describe a role for PET in patients with neuroblastoma (NB). Patients and Methods: In 51 patients with high-risk NB, 92 PET scans were part of a staging evaluation that included iodine-123 or iodine-131 metaiodobenzyl- guanidine (MIBG) scan, bone scan, computed tomogra- phy (and/or magnetic resonance imaging), urine cate- cholamine ...

Dopamine Induces Autophagic Cell Death and -Synuclein Increase in Human Neuroblastoma SH-SY5Y Cells Cristina Gómez-Santos, 1 Isidre Ferrer, 2 Antonio F. Santidrián, 1 Marta Barrachina, 2 Joan Gil, 1 and Santiago Ambrosio 1 * 1 Unitat de Bioquim Departament de Cie`ncies Fisiolo`giques II, Universitat de Barcelona, Barcelona, Spain 2 Unitat de Neuropatologia, Departament Biologia Cel-lular i Anatomia Patolo`gica, Universitat de Barcelona, Barcelona, Spain Free cytoplasmic dopamine may be involved in the gen- esis of neuronal degeneration in Parkinson’s disease and other such diseases. We used SH-SY5Y human neuro- blastoma cells to study the effect of dopamine on cell death, activation of stress-induced pathways, and ex- pression of -synuclein, the characteristic ...

Olfactory neuroblastomas are relatively uncommon tu- mors that originate from the olfactory epithelium in the upper nasal cavity in the region of the cribriform plate. Although some controversy persists, their primary modal- ity of therapy is resection, usually performed by an an- terior craniofacial approach and with the provision of ad- juvant radiation therapy. 29 In addition, recent experience suggests that there may be a therapeutic role for adjuvant chemotherapy. 4 A variety of other neoplastic lesions occur in the same anatomical region as ONs. Tumors such as NEC, melanoma, sinonasal lymphoma, SNUC, and pitu- itary adenoma can all present with similar clinical, histo- logical, and radiological features as ON. The prognosis and therapeutic modalities for these different tumors are variable, and physicians treating neoplasms arising from the paranasal sinus region ...

3 body’s white cells — a hypothesis that has since been proven at the bench — and is now being tested at the bedside. In animal studies Cheung used purified glucan taken from barley and oats for oral administration. “We tested their effec- tiveness in laboratory mice carrying neurob- lastoma tumors,” Che- ung explains. “When used alone it was not effective. But when we used it along with the anti-GD2 mon- oclonal anti- body 3F8, tumor growth was nearly total- ly suppressed.” Cheung believes that when the glu- can molecule arrives at certain parts of the gastrointestinal tract, it attach- es to “receptor cells,” activating them to release cancer-fighting leukocytes to attack the tumor. This action, however, is dependent on the antibodies that lay down certain proteins on the surface of tumor cells. “They are natural ...

Neuroblastoma Incidence in Illinois: 1986-1997 Neuroblastoma is one of the most commonly diagnosed solid tumors in young children. The disease originates from neural cell ectoderm that gives rise in the developing fetus to the sympathetic nervous system, the adrenal medulla, and the adrenergic and cholinergic neuroblasts along the sympathetic chain and cranial ganglia. The annual incidence rate among children under 15 years of age in the United States is nine to 11 cases per million (1). In Illinois, according to data reported to the Illinois State Cancer Registry from 1986 through 1997, the annual incidence rate was 10.5 per million. Neuroblastoma incidence at state and county levels has been reported previously for 1986 through 1994 by the Illinois State Cancer Registry (2). The current study updates the previous report through adding three more recent years of data to the original analysis. During the 12-year period ...

Neuroblastoma – the basics Disclaimer: This fact sheet is for education purposes only. Please consult with your doctor or other health professional to make sure this information is right for your child. This information sheet will tell you in simple terms: What is a neuroblastoma ? Why did the neuroblastoma happen? What problems does neuroblastoma cause? How did we find the neuroblastoma ? How do we treat neuroblastoma ? What is a neuroblastoma ? A neuroblastoma is a type of cancer - a lump of cells (tumour) which should not be there. Neuroblastoma is a cancer that only children get. It starts from primitive (very simple) nerve cells found in areas where nerves join together. The most common areas where these are found are in the abdomen (especially the adrenal gland), chest and neck. Why did the neuroblastoma happen? In most cases, we do not know why children get ...